微塑料和三氯生对斑马鱼的神经毒性效应研究  

Study on neurotoxic effects of microplastics and triclosan on the zebrafish

作　　者：安浩 张宴[2] AN Hao;ZHANG Yan(China MCC5 Group Corp.LTD.,Chengdu 610000,China;School of Environment,Nanjing University,Nanjing 210000,China)

机构地区：[1]中国五冶集团有限公司,四川成都610000 [2]南京大学环境学院,江苏南京210000

出　　处：《能源环境保护》2023年第4期131-139,共9页Energy Environmental Protection

基　　金：国家自然科学基金(51508263)。

摘　　要：选用斑马鱼为模式生物,探究3种微塑料(PP、PE、PVC)和三氯生(TCS)联合暴露对斑马鱼的神经毒性效应。结果表明:微塑料(MPs)可以作为TCS进入生物体内的载体,MPs和TCS联合暴露毒性主要为协同作用;与TCS相比,TCS+PP、TCS+PE和TCS+PVC组中SOD分别降低了10.3%、12.4%和7.9%,AchE活力分别降低了10.8%、1.5%和6.1%,5-HT含量分别提高了83.2%、37.7%和59.3%(p<0.05);与TCS+PE相比,TCS+PP组5-HT含量提高了33.1%,具有显著性差异(p<0.05),PP-MPs和TCS联合暴露产生了更强的神经毒性;代谢组分析发现TCS+PP复合组诱导98个特异性变化的代谢物,这些代谢物通过影响氨基酸代谢、甘油磷脂代谢、鞘脂代谢、花生四烯酸代谢和神经活性配体受体相互作用等代谢过程,对脑产生更严重的神经毒性。Zebrafish was chosen as a model organism to explore the neurotoxic effects of combined exposure to three microplastics(PP,PE,PVC)and triclosan(TCS).The results showed that microplastics(MPs)could be used as the carriers of TCS in vivo,and the combined toxicity of MPs and TCS was mainly synergistic.Compared with TCS,SOD in TCS+PP,TCS+PE and TCS+PVC groups decreased by 10.3%,12.4% and 7.9%,and AchE activity decreased by 10.8%,1.5% and 6.1%,respectively.The 5-HT content was increased by 83.2%,37.7%and 59.3%(p<0.05),respectively.Compared with TCS+PE,the 5-HT content in TCS+PP group was increased by 33.1%,showing a significant difference(p<0.05).Combined exposure of PP-MPS and TCS produced stronger neurotoxicity.Metabolome analysis showed that the TCS+PP complex group induced 98 metabolites with specific changes,and these metabolites produced more serious neurotoxicity to the brain by affecting amino acid metabolism,glycerophospholipid metabolism,sphingolipid metabolism,arachidonic acid metabolism and neuroactive ligand receptor interaction and other metabolic processes.

关 键 词：微塑料 三氯生 生物富集 联合毒性 

分 类 号：X703[环境科学与工程—环境工程]