绿鲍(Haliotis fulgens)线粒体基因组全序列测定及分析  

Sequencing and analysis of the complete mitochondrial genome of green abalone(Haliotis fulgens)

作　　者：郭睿 张善霹[1,2] 蔡雷鸣 杨小强[1,2] 王伟 江小斌[1,2] 林钦 林枫[3] 林哲龙 Guo Rui;Zhang Shanpi;Cai Leiming;Yang Xiaoqiang;Wang Wei;Jiang Xiaobin;Lin Qin;Lin Feng;Lin Zhelong(Marine and Fisheries Technology Research Center,Institute of Oceanography of Fuzhou,Fuzhou 350108,China;Fuzhou Ocean and Fisheries Technology Center,Fuzhou 350007,China;Fujian Lianjiang Guanwu Marine Products and Development Co.,Ltd.,Lianjiang 350511,China)

机构地区：[1]福州海洋研究院海洋与渔业技术研究中心,福建福州350108 [2]福州市海洋与渔业技术中心,福建福州350007 [3]福建省连江县官坞海产开发有限公司,福建连江350511

出　　处：《海洋学报》2023年第5期64-78,共15页

基　　金：福州海洋研究院项目(2021F12);福建省科技厅项目(2021S0010)。

摘　　要：为高效鉴别鲍属物种和更好地管理和保护鲍种质资源,本研究通过高通量测序获得了养殖绿鲍(Haliotis fulgens)稚贝的线粒体基因组全序列,并对其序列和结构特征进行分析。结果表明,绿鲍线粒体基因组全长17 041 bp,含有37个编码基因,其中蛋白质编码基因13个、t RNA基因22个、r RNA基因2个。13个蛋白质编码基因均以AUG为起始密码子,以UAG或UAA为终止密码子。除t RNA-Ser^((AGN))外的21个tRNA基因可折叠成典型三叶草结构。分析发现t RNA-Glu和COX3间存在富含A+T的非编码区,其内含有2个带回文序列的发卡结构。基于已报道的10个鲍属线粒体基因组全序列构建系统发育树,发现绿鲍与皱纹盘鲍(Haliotis discus hannai)、红鲍(Haliotis rufescens)、黑鲍(Haliotis cracherodii)聚为一支。将绿鲍与皱纹盘鲍13个线粒体编码蛋白的结构域比较,发现二者ND2、ND4的跨膜结构域数量存在差异,这是否与二者的高温耐受性差异有关,有待进一步研究。总之,绿鲍线粒体基因组全序列的首次获取和分析,丰富了鲍类细胞遗传信息,为分类、种质鉴定与种质资源保护提供了基础数据和参考。To identify abalone species effectively for better management and protection of abalone germplasm re-sources,we have obtained the complete mitochondrial genome of juvenile green abalone(Haliotis fulgens)through high-throughput sequencing,and its sequences and structural characteristics are analyzed accordingly.The results show that the mitogenome of H.fulgens is 17041 bp in total length and encoded 37 genes,including 13 protein cod-ing genes,22 transfer RNA(tRNA)genes and 2 ribosomal RNA(rRNA)genes.The 13 protein coding genes used AUG as the starting codon and UAA or UAG as the termination codon.Twenty-one tRNA genes other than tRNA-Ser^((AGN))could be folded into a typical cloverleaf structure.There is a rich A+T non-coding region between tRNA-Glu and COX3,with two hairpin structures containing palindromes in it.A phylogenetic tree was constructed based on the reported complete mitochondrial genome sequences of Haliotis genus species,and phylogenetic analysis shows that H.fulgens is clustered with H.discus hannai,H.rufescens and H.cracherodii.Comparing the domains of 13 proteins of mitogenome of H.fulgens and H.discus hannai,we found the number of transmembrane domains of ND2 or ND4 dehydrogenase subunits were different in them.Whether this is related to the difference of high tem-perature tolerance between them needs further study.In brief,the first acquisition and analysis of the complete mi-tochondrial genome of H.fulgens has enriched the abalone cellular genetic information,and provided basic data and references for abalone species classification,germplasm identification,and protection of germplasm resources of H.fulgens as well.

关 键 词：绿鲍 线粒体基因组 基因组组成 系统发育 结构域 

分 类 号：P735[天文地球—海洋生物学] Q959.212[天文地球—海洋科学]