Trx-1在脓毒症小鼠内皮细胞铁死亡中的表达  

Expression of Trx-1 in Ferroptosis of Endothelial Cells in Sepsis

作　　者：刘锋 马大珍 刘茜 杨梅[1] 刘楠 朱国际[1] LIU Feng;MA Da-zhen;LIU Qian;YANG Mei;LIU Nan;ZHU Guo-ji(Children's Hospital of Soochow University,Suzhou,Jiangsu,215003,China)

机构地区：[1]苏州大学附属儿童医院,江苏苏州215003

出　　处：《中国血液流变学杂志》2023年第1期6-10,共5页Chinese Journal of Hemorheology

基　　金：苏州市科技局民生科技-医疗卫生应用基础研究(SYS2020162)。

摘　　要：目的研究脓毒症小鼠血管内皮细胞是否存在铁死亡以及硫氧还蛋白-1(thioredoxin-1,Trx-1)在这一过程中的表达。方法采用盲肠结扎穿孔术(cecal ligation and puncture,CLP)构建脓毒症小鼠模型。将动物随机分成3组:假手术组(Sham组)、CLP组、铁死亡抑制剂组(Fer-1组)。观察12h后,取外周血制备血清,采用ELISA法检测各组小鼠外周血中Trx-1、血管内皮生长因子(VEGF)及内皮素-1(ET-1)的表达。提取及培养小鼠原代内皮细胞,应用荧光探针技术测定内皮细胞内Fe^(2+)及ROS的水平,试剂盒法测定内皮细胞内谷胱甘肽(glutathione,GSH)及丙二醛(malondialdehyde,MDA)的水平。结果与Sham组相比,CLP组内皮细胞内GSH含量降低,Fe^(2+)、ROS、MDA含量升高,Fer-1预处理能够缓解这一过程;与Sham组相比,CLP组Trx-1、ET-1及VEGF含量升高,Fer-1组较CLP组降低。结论脓毒症小鼠内皮细胞存在铁死亡,Trx-1在脓毒症铁死亡中高表达。Objective To study whether there is ferroptosis in endothelial cells of septic mice and the expression of thioredoxin-1(Trx-1)in this process.Methods The septic mouse model was established by cecal ligation and puncture(CLP).The animals were randomly divided into three groups:sham operation group,CLP group and ferrostatin inhibitor group.After 12 hours of observation,peripheral blood was taken to prepare serum,the levels of ET-1,VEGF and Trx-1 in peripheral blood of mice in each group were measured by ELISA kit.Extraction and culture of primary mouse endothelial cells,the levels of ROS and Fe^(2+)in endothelial cells were measured by fluorescence probe technique.The levels of malondialdehyde(MDA)and glutathione(GSH)in endothelial cells were measured by kit method.Results Compared with Sham group,the GSH content in endothelial cells was reduced and the Fe^(2+),ROS and MDA contents were increased in the CLP group.Fer-1 pretreatment could alleviate this process.Compared with Sham group,the Trx-1,ET-1 and VEGF contents were increased in the CLP group.Conclusion There is ferroptosis in endothelial cells of septic mice,and Trx-1 is highly expressed in septic ferroptosis.

关 键 词：Trx-1 脓毒症 内皮细胞 铁死亡 

分 类 号：R631[医药卫生—外科学]