异叶三宝木的二萜成分及抗菌活性研究  

Chemical Constituents and Antibacterial Activities of Trigonostemon heterophyllus

作　　者：岑祥妮 范艺灜 刘浩文 李佳莹 王川林 刘平怀[1] CEN Xiangni;FAN Yiying;LIU Haowen;LI Jiayin;WANG Chuanlin;LIU Pinghuai(School of Chemical Engineering and Technology,Hainan University,Haikou 570228,China)

机构地区：[1]海南大学化学工程与技术学院,海口570228

出　　处：《热带亚热带植物学报》2023年第4期602-606,共5页Journal of Tropical and Subtropical Botany

基　　金：海南省重点研发计划项目(ZDYF2020169)资助。

摘　　要：为研究异叶三宝木(Trigonostemon heterophyllus)的二萜成分及其抗菌活性,采用硅胶柱层析、凝胶柱层析、高效液相色谱对萃取物进行分离纯化,结合现代波谱技术对所得化合物进行结构鉴定,并通过牛津杯法和2倍稀释法检测化合物对革兰氏阴性菌大肠杆菌(Escherichia coli)以及革兰氏阳性菌肺炎双球菌(Pneumococcus)的生长抑制活性和MIC值。从异叶三宝木中分离得到了6个化合物,包括5个二萜类,1个木脂素类,分别鉴定为trigonochinene E(1)、neoboutomannin(2)、6,9-O-dedimethyltrigonostemone(3)、stelltian B(4)、3,4-secosonderianol(5)、biondinin A(6)。化合物1、2、3、5对大肠杆菌有抑制作用,MIC值分别为9.375、18.75、18.75、18.75μg/mL。化合物2、3、4、6为首次从该种植物中分离得到,化合物4为首次从该属植物中分离得到。除化合物4外,其他化合物都有一定的抗菌活性,且化合物1、2、3对大肠杆菌抑制作用强于阳性对照硫酸卡那霉素。To understand the chemical constituents and antibacterial activities of Trigonostemon heterophyllus,six compounds,including five diterpenoids and one lignan,were isolated and purified by silica gel column chromate-graphy,gel column chromatography and high performance liquid chromatography.Based on spectroscopic data,their structures were identified as trigonochinene E(1),neoboutomannin(2),6,9-O-dedimethyl trigonostemone(3),stelltian B(4),3,4-secosonderianol(5),biondinin A(6).The inhibitory activity and MIC values of compounds against a Gram-negative bacterium,Escherichia coli,and a Gram-positive bacterium,Pneumococcus,were detected by Oxford Cup method and double dilution method.Compounds 1,2,3 and 5 had inhibitory effects on Escherichia coli with MIC values of 9.375,18.75,18.75 and 18.75μg/mL,respectively.Compounds 2,3,4,and 6 were isolated from T.heterophyllus for the first time,and compound 4 was isolated from Trigonostemon genus for the first time.Except compound 4,all the other compounds had certain antibacterial activity,and compounds 1,2 and 3 had stronger inhibitory effect on Escherichia coli than Kanamycin sulfate positive control.

关 键 词：异叶三宝木 二萜类 大肠杆菌 肺炎双球菌 

分 类 号：R284.1[医药卫生—中药学]