干旱胁迫下野生大豆水通道蛋白GsPIP2;7功能研究  

Functional Characterization of Aquaporin GsPIP2;7 in Wild Soybean With Drought Stress

作　　者：王宇[1] 赵圆 王艳丽 俎天娇 司增志 孙伟明 乔亚科[1] 张锴[1] WANG Yu;ZHAO Yuan;WANG Yanli;ZU Tianjiao;SI Zengzhi;SUN Weiming;QIAO Yake;ZHANG Kai(Hebei Key Laboratory of Crop Stress Biology(inPreparation),Hebei Normal University of Science and Technology,Qinhuangdao,Hebei 066004)

机构地区：[1]河北科技师范学院,河北省作物逆境生物学重点实验室(筹),河北秦皇岛066004

出　　处：《核农学报》2023年第7期1315-1322,共8页Journal of Nuclear Agricultural Sciences

基　　金：河北省重点研发计划项目(19226365D);河北省青年拔尖人才项目(BJ2019051);河北省大豆产业技术体系(326-0702-JSNTKSF);河北省科技厅现代种业科技创新专项(21326313D)。

摘　　要：水通道蛋白(AQPs)是植物中重要的水分转运蛋白,在植物对抗干旱胁迫中发挥着重要功能。为明确野生大豆水通道蛋白在植株抗旱反应中的分子功能,本研究对野生大豆(Glycine soja)中的水通道蛋白的蛋白结构及其在干旱胁迫下的表达模式进行分析,并对筛选的AQPs基因功能进行验证。研究鉴定了野生大豆中69个AQPs蛋白,其中10个在干旱胁迫的高耐野生大豆材料中表达差异达到显著水平。筛选到GsPIP2;7在干旱胁迫后与对照相比显著上调,克隆GsPIP2;7到过表达载体中并侵染转化拟南芥(Arabidopsis thaliana),通过转基因后代筛选获得了稳定表达的T3代转基因拟南芥。与野生型拟南芥相比,转基因植株在干旱胁迫下根系总长度、叶干重、根干重、根冠比和单株叶面积显著增加,过氧化物酶(POD)、过氧化氢酶(CAT)和超氧化物歧化酶(SOD)活性显著提升,丙二醛(MDA)含量显著降低,表现出较强的耐旱能力。本研究结果说明GsPIP2;7对拟南芥耐旱具有正向调控作用,为明确野生大豆耐旱机制奠定了理论基础。Aquaporins(AQPs)are important water transport proteins in plants,which play an important role in resistance to drought stress.To clarify the molecular function of AQPs of wild soybean in response to drought resistance,in this study,the protein structures and the expression patterns of AQPs in wild soybean(Glycine soja)were analyzed under drought stress,and the gene functions of selected AQPs were identified.The results showed that there were a total of 69 AQPs proteins existed in wild soybean,of which 10 proteins were significantly different expressed in high tolerance wild soybean lines under drought stress.GsPIP2;7 was significantly up-regulated under drought stress.Then GsPIP2;7 gene was cloned into the overexpression vector and transformed into Arabidopsis thaliana.The T3 generation of transgenic Arabidopsis thaliana lines with stable expression of GSPIP2;7 were obtained by identified the transgenic progeny.Compared with the wild-type Arabidopsis thaliana,the total root length,leaf dry weight,root dry weight,root shoot ratio and leaf area per plant of transgenic plants were significantly increased under drought stress.The activities of peroxidase(POD),catalase(CAT)and superoxide dismutase(SOD)were significantly increased,and the content of malondialdehyde(MDA)was significantly decreased.The results showed that GsPIP2;7 had positive function in the regulation of drought tolerance in Arabidopsis,which established theoretical basis for clarifying the drought tolerance mechanism in wild soybean.

关 键 词：干旱胁迫 野生大豆 水通道蛋白 GsPIP2 7 

分 类 号：S565.1[农业科学—作物学]