金银莲花横走茎形态发育的生理特性及繁育研究  

Physiological Characteristics and Breeding on Morphological Development of Nymphoides indica Transverse Stem

作　　者：谢欢 柳贤 陈斯 杨自云[1] 刘艳艳 艾星梅[1] Xie Huan;Liu Xian;Chen Si;Yang Ziyun;Liu Yanyan;Ai Xingmei(College of Landscape Architecture and Horticulture,Southwest Forestry University/Landscape Engineering Research Center in Southwest China,National Forestry and Grasseland Administraton,Kunming Yunnan 650233,China;Agricultural Comprehensive Service Center of XiazhuangTown,Dali Yunnan672105,China)

机构地区：[1]西南林业大学园林园艺学院,国家林业和草原局西南风景园林工程中心,云南昆明650233 [2]大理州祥云县下庄镇农业综合服务中心,云南大理672105

出　　处：《西南林业大学学报（自然科学）》2023年第4期47-58,共12页Journal of Southwest Forestry University：Natural Sciences

基　　金：云南省教育厅科学研究基金研究生项目(2021Y254)资助;云南省教育厅科学研究基金项目(2019J0197,2018JS334)资助。

摘　　要：以金银莲花横走茎为材料,采用石蜡切片技术观察其形态发育过程,结合叶片不同部位的生理差异,研究茎节发育的生理机制及分株栽培方式。结果表明:茎节起始于幼嫩茎最外轮维管束周围的薄壁细胞,不断分裂增加形成原基细胞团,分化后形成大量叶片、根系和花序。茎节发育初期,金银莲花叶片的叶绿素含量、蛋白质含量以及茎节生长点的可溶性糖含量处于较高水平;茎节生长点的淀粉和蔗糖含量先升后降,不同组织的碳水化合物与抗氧化酶活性均表现为茎节生长点>叶片>叶柄;表明酶活性与碳水化合物之间存在协同作用。不同分株方式的横走茎均可在塘泥和河沙基质中形成新植株,其中塘泥+1/2分株法为金银莲花繁殖的较好方式。研究结果可为金银莲花的栽培繁育提供参考。We observed the process of morphological development of Nymphoides indica transverse stems by paraffin section technique,and combined with the different in physiological characteristics of different parts of leaves to study the physiological mechanism and ramet cuitivation methods of its stem development.The results showed that the stem node started from the parenchyma cells that located in the adaxial part near the outer ring of vascular bundle,which divided continuously to form the primodial cells and finally differentiated to form the leaves,roots and inflorescences.In the early stage of stem growth point formed,the chlorophyll content and soluble protein of the leaves and sugar content of nodal growth points of N.indica were at a high level;while the starch and sucrose content of the nodal growth points increased first and then decreased.Overall,the carbohydrate content and antioxidant enzyme activities of different tissues showed that stem node growing point>leaf>petiole,and there was a synergistic effect between the enzymatic activity and carbohydrate.All of the transverse stem have formed new plants in pond mud and river sand by different ramets propagation,of which pond mud+1/2 ramet method is a better way to expand propagation.The results can provide reference for the cultivation and propagation of N.indica.

关 键 词：金银莲花 横走茎 形态解剖 生理特性 分株繁育 

分 类 号：S68[农业科学—观赏园艺]