High-resolution anatomical and spatial transcriptome analyses reveal two types of meristematic cell pools within the secondary vascular tissue of poplar stem  

作　　者：Juan Du Yichen Wang Wenfan Chen Mingling Xu Ruhong Zhou Huixia Shou Jun Chen 

机构地区：[1]State Key Laboratory of Plant Physiology and Biochemistry,College of Life Sciences,Zhejang University,866Yu Hangtang Road,Hangzhou,Zhejiang 310058,China [2]MOE Key Laboratory of Biosystems Homeostasis&Protection,College of Life Sciences,Zhejang University,866 Yu Hangtang Road,Hangzhou,Zhejang 310058,China [3]Institute of Quantitative Biology,College of Life Sciences,Zhejiang University,Hangzhou,Zhejiang 310058,China [4]Hangzhou Botanical Garden,Taoyuanling Road,Hangzhou,Zhejiang 310013,China

出　　处：《Molecular Plant》2023年第5期809-828,共20页分子植物（英文版）

基　　金：supported by the National Natural Science Foundation of China(32071792)to J.D.,Zhejiang University;National Key Program on 2016YFD0600103 to J.D.,Zhejiang University;The Key program of the National Science Foundation of Zhejiang province(LZ22C160002)to J.D.,Zhejiang University;National Key R&D Program of China(2021YFF1200404)to R.H.Z.,Zhejiang University;Starry Night Science Fund of Zhejiang University Shanghai Institute for Advanced Study(SNZJU-SIAS-003/011)to R.H.Z.,Zhejiang University.

摘　　要：The secondary vascular tissue emanating from meristems is central to understanding how vascular plants such as forest trees evolve,grow,and regulate secondary radial growth.However,the overall molecular characterization of meristem origins and developmental trajectories from primary to secondary vascular tissues in woody tree stems is technically challenging.In this study,we combined high-resolution anatomic analysis with a spatial transcriptome(ST)technique to define features of meristematic cells in a developmental gradient from primary to secondary vascular tissues in poplar stems.The tissue-specific gene expression of meristems and derived vascular tissue types were accordingly mapped to specific anatomical domains.Pseudotime analyses were used to track the origins and changes of meristems throughout the development from primary to secondary vascular tissues.Surprisingly,two types of meristematic-like cell pools within secondary vascular tissues were inferred based on high-resolution microscopy combined with ST,and the results were confirmed by in situ hybridization of,transgenic trees,and single-cell sequencing.The rectangle shape procambium-like(PCL)cells develop from procambium meristematic cells and are located within the phloem domain to produce phloem cells,whereas fusiform shape cambium zone(CZ)meristematic cells develop from fusiform metacambium meristematic cells and are located inside the CZ to produce xylem cells.The gene expression atlas and transcriptional networks spanning the primary transition to secondary vascular tissues generated in this work provide new resources for studying the regulation of meristem activities and the evolution of vascular plants.A web server(https://pgx.zju.edu.cn/stRNAPal/)was also established to facilitate the use of ST RNA-seq data.

关 键 词：poplar stem primary and secondary meristem primary and secondary vascular tissue spatial transcriptome single-cell sequencing 

分 类 号：Q94[生物学—植物学]