The WAK-like protein RFO1 acts as a sensor of the pectin methylation status in Arabidopsis cell walls to modulate root growth and defense  

作　　者：Apolonio I.Huerta Gloria Sancho-Andre´s Juan Carlos Montesinos Javier Silva-Navas Sole`ne Bassard Corinne Pau-Roblot Christopher Kesten Rudolf Schlechter Susanne Dora Temurkhan Ayupov Je´rome Pelloux^ Julia Santiago Clara Sa´nchez-Rodrı´guez 

机构地区：[1]ETH Zurich,Institute of Molecular Plant Biology(D-BIOL),Zurich,Switzerland [2]University of Lausanne,Department of Plant Molecular Biology,Lausanne,Switzerland [3]UMRT INRAE 1158 BioEcoAgro–BIOPI Biologie des Plantes et Innovation,Universite´de Picardie,33 Rue St Leu,80039 Amiens,France [4]Present address:Lonza AG,VispVis,Switzerland [5]Present address:Institute of Microbiology,Freie Universitat Berlin,Berlin,Germany [6]Present address:Institute of Molecular and Clinical Ophthalmology-Basel,Basel,Switzerland [7]Present address:Centro de Biotecnologı´a y Geno´mica de Plantas,Universidad Polite´cnica de Madrid(UPM)–Instituto Nacional de Investigacio´n y Tecnologı´a Agraria y Alimentaria(INIA/CSIC),Campus de Montegancedo UPM,28223 Pozuelo de Alarco´n,Spain

出　　处：《Molecular Plant》2023年第5期865-881,共17页分子植物（英文版）

基　　金：supported by the Swiss National Foundation and the Heinz Imhof Foundation to C.S.R.(2-72160-16 to A.I.H.,SNF 31003A_163065/1 and SNF 310030_184769 to G.S.);the Peter und Traudl Engelhorn Stiftung to C.K.,and the European Research Council(ERC)grant agreement no.716358;the Fondation Philanthropique Famille Sandoz to J.S.

摘　　要：Most organisms adjust their development according to the environmental conditions.For the majority,this implies the sensing of alterations to cell walls caused by different cues.Despite the relevance of this process,few molecular players involved in cell wall sensing are known and characterized.Here,we show that the wall-associated kinase-like protein RESISTANCE TO FUSARIUM OXYSPORUM 1(RFO1)is required for plant growth and early defense against Fusarium oxysporum and functions by sensing changes in the pectin methylation levels in the cell wall.The RFO1 dwell time at the plasma membrane is affected by the pectin methylation status at the cell wall,regulating MITOGEN-ACTIVATED PROTEIN KINASE and gene expression.We show that the extracellular domain of RFO1 binds de-methylated pectin in vitro,whose distribution in the cell wall is altered during F.oxysporum infection.Further analyses also indicate that RFO1 is required for the BR-dependent plant growth alteration in response to inhibition of pectin de-methyl-esterase activity at the cell wall.Collectively,our work demonstrates that RFO1 is a sensor of the pectin methylation status that plays a unique dual role in plant growth and defense against vascular pathogens.

关 键 词：alterations walls PECTIN 

分 类 号：Q94[生物学—植物学]