Suppression of ETI by PTI priming to balance plant growth and defense through an MPK3/MPK6-WRKYs-PP2Cs module  

作　　者：Dacheng Wang Lirong Wei Ting Liu Jinbiao Ma Keyi Huang Huimin Guo Yufen Huang Lei Zhang Jing Zhao Kenichi Tsuda Yiming Wang 

机构地区：[1]Key Laboratory of Biological Interactions and Crop Health,Department of Plant Pathology,Nanjing Agricultural University,Nanjing 210095,China [2]Key Laboratory of Integrated Management of Crop Disease and Pests,Ministry of Education,Nanjing Agricultural University,Nanjing,China [3]State Key Laboratory of Agricultural Microbiology,Hubei Hongshan Laboratory,Hubei Key Lab of Plant Pathology,College of Plant Science and Technology,Huazhong Agricultural University,Wuhan,China

出　　处：《Molecular Plant》2023年第5期903-918,共16页分子植物（英文版）

基　　金：supported by grants from the National Key Research and Development Project(2022YFE0198100);National Natural Science Foundation of China(32172420);Natural Science Foundation of Jiangsu Province(SBK20220085);Fundamental Research Funds for the Central Universities(KYXK202009,ZJ21195012);the Startup Fund for Distinguished Scholars from Nanjing Agricultural University(to Y.W.).

摘　　要：Pattern-triggered immunity(PTI)and effector-triggered immunity(ETI)are required for host defense against pathogens.Although PTI and ETI are intimately connected,the underlying molecular mechanisms remain elusive.In this study,we demonstrate that flg22 priming attenuates Pseudomonas syringae pv.tomato DC3000(Pst)AvrRpt2-induced hypersensitive cell death,resistance,and biomass reduction in Arabidopsis.Mitogen-activated protein kinases(MAPKs)are key signaling regulators of PTI and ETI.The absence of MPK3 and MPK6 significantly reduces pre-PTI-mediated ETI suppression(PES).We found that MPK3/MPK6 interact with and phosphorylate the downstream transcription factor WRKY18,which regulates the expression of AP2C1 and PP2C5,two genes encoding protein phosphatases.Furthermore,we observed that the PTI-suppressed ETI-triggered cell death,MAPK activation,and growth retardation are significantly attenuated in wrky18/40/60 and ap2c1 pp2c5 mutants.Taken together,our results suggest that the MPK3/MPK6-WRKYs-PP2Cs module underlies PES and is essential for the maintenance of plant fitness during ETI.

关 键 词：PAMP-triggered immunity effector-triggered immunity MAPK WRKY18 protein phosphatase 

分 类 号：Q94[生物学—植物学]