OSA患者性激素与HIF-1α/NF-κB/IL-6信号通路的关系分析  

Analysis of the Relationship Between Sex Hormones and HIF-1α/NF-κB/IL-6 Signaling Pathway in Patients With OSA

作　　者：谢涵 尹虹雷[1] 林庆艳[1] 徐英[1] XIE Han;YIN Honglei;LIN Qingyan;XU Ying(Department of Respiratory and Critical Care Medicine,Heilongjiang Provincial Hospital,Harbin Heilongjiang 150036,China)

机构地区：[1]黑龙江省医院南岗院区呼吸与危重病科,黑龙江哈尔滨150036

出　　处：《中国卫生标准管理》2023年第13期123-127,共5页China Health Standard Management

基　　金：黑龙江省卫生健康委科研课题(20220303020997)。

摘　　要：目的分析阻塞性睡眠呼吸暂停(obstructive sleep apnea,OSA)患者性激素与缺氧诱导因子1α(hypoxia inducible factor-1α,HIF-1α)/NF-κB/IL-6信号通路的关系。方法选取黑龙江省医院2019年1月—2022年1月的40例OSA患者进行分析,同时选取40例健康体检者作为对照组进行对比,给予OSA患者持续气道正压通气(continuous postive airway pressure,CPAP)治疗3个月,在治疗前后分别于晨起空腹采血,Eillisa法检测各组血清卵泡刺激素(follicle stimating hormone,FSH)、黄体生成素(luteinizing hormone,LH)、睾酮(testosterone,T)、雌二醇(estradiol,E2)、白细胞介素-6(interleukin-6,IL-6)、核转录因子(nuclear transcription factor-kappa B,NF-κB)水平,HIF-1α的水平并记录。结果OSA患者治疗前血中HIF-1α、NF-κB及IL-6水平较对照组高,OSA患者睾酮水平低,同时FSH、LH增高,E2低,但差异无统计学意义(P>0.05)。OSA患者经CPAP治疗后HIF-1α及NF-κB及IL-6水平较治疗前下降,睾酮水平上升,FSH、LH、E2无明显变化。结论HIF-1α可能通过介导NF-κB/IL-6信号通路导致OSA患者下丘脑-垂体-性腺轴功能紊乱,通过治疗OSA,可减少炎症反应水平,从而减少OSA对下丘脑-垂体-性腺轴的影响,恢复性激素水平。Objective To analyze the relationship between sex hormones and HIF-1α/NF-κB/IL-6 signaling pathway in patients with obstructive sleep apnea(OSA).Methods A total of OSA patients in Heilongjiang Provincial Hospital from January 2019 to January 2022 were selected for analysis,while 40 healthy subjects were selected as the control group for comparison,patients with OSA were treated with continuous postive airway pressure(CPAP)for 3 months.Fasting blood was collected in the morning before and after treatment,Eillisa method was used to detect follicle stimating hormone(FSH),luteinizing hormone(LH),testosterone(T),estradiol(E2),interleukin-6(IL-6),nuclear transcription factor-kappa B(NF-κB),and the level of HIF-1α.Results The level of HIF-1αin OSA patients before treatment was higher,NF-κB and IL-6 were higher compared with the control group,and testosterone in OSA patients was lower.Meanwhile,the level of FSH and LH were increased,E2 was lower,but there was no statistical significance(P>0.05).After CPAP treatment,the level of HIF-1α,NF-κB and IL-6 in OSA patients were decreased,while testosterone was increased,but FSH,LH,E2 did not change significantly.Conclusion HIF-1αmay cause hypothalamicpituitary-gonad axis dysfunction in OSA patients by mediating NF-κB/IL-6 signaling pathway.By treating OSA,the level of inflammatory response can be reduced,thus reducing the influence of OSA on the hypothalamic-pituitary-gonad axis and restoring the level of sex hormones.

关 键 词：阻塞性睡眠呼吸暂停 性激素 缺氧诱导因子1Α 性腺轴 炎症反应 睾酮 

分 类 号：R681[医药卫生—骨科学]