莲子草假隔链格孢聚酮合成酶基因的筛选、克隆及生物信息学分析  

Screening,cloning and bioinformatics analysis of polyketide synthase genes in the Nimbya alternantherae of Alternanthera philoxeroides

作　　者：夏广泽 Xia Guangze(Linyi University,Shandongg 276000,China)

机构地区：[1]临沂大学,山东临沂276000

出　　处：《植物检疫》2023年第4期1-7,共7页Plant Quarantine

摘　　要：通过对莲子草假隔链格孢转录组测序中聚酮合成酶基因的表达分析,发现编号为Unigene0007998的基因在莲子草假隔链格孢中的表达量远高于其他聚酮合成酶,推测该基因与莲子草假隔链格孢的致病力相关。为进一步了解该基因的功能,本研究克隆了该基因并对其编码的蛋白进行了生物信息学分析。结果表明Unigene0007998编码的蛋白由2089个氨基酸组成,是一种稳定的、亲水性分泌蛋白,不存在跨膜结构。By analyzing the expression of PKS genes in the transcriptome sequencing results of Nimbya alternantherae,it was found that the expression of Unigene0007998 was much higher than that of other PKS genes.It was speculated that Unigene0007998 gene was related to the pathogenicity of N.alternatherae.In order to further understand the function of Unigene0007998 gene,the gene was cloned and the protein coded by Unigene0007998 was analyzed by bioinformatic methods.The results showed that the protein coded by Unigene0007998 consisted of 2089 amino acids,and was a stable,hydrophilia,secreted protein without transmembrane structure.

关 键 词：空心莲子草 莲子草假隔链格孢 聚酮合成酶 致病力 

分 类 号：S476[农业科学—农业昆虫与害虫防治]