基于HPLC-ELSD指纹图谱和多成分定量的浙贝母与湖北贝母质量差异研究  

Quality difference between Fritillariae Thunbergii Bulbus and Fritillariae Hupehensis Bulbus based on HPLC-ELSD fingerprints and multi-component quantitative analysis

作　　者：梁月仪 李振雨 吕渭升 卢晓莹 杨洁 刘晓霞 位翠杰 孙冬梅 LIANG Yue-yi;LI Zhen-yu;LYU Wei-sheng;LU Xiao-ying;YANG Jie;LIU Xiao-xia;WEI Cui-jie;SUN Dong-mei(Guangdong Yifang Pharmaceutical Co.,Ltd.;Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula,Foshan 528244,China)

机构地区：[1]广东一方制药有限公司 [2]广东省中药配方颗粒企业重点实验室,佛山528244

出　　处：《天然产物研究与开发》2023年第7期1101-1111,共11页Natural Product Research and Development

基　　金：佛山市应急科技攻关专项(2020001000206);国家工业和信息化部2019年产业技术基础公共服务平台项目(2019-00902-1-2)。

摘　　要：建立浙贝母、湖北贝母HPLC-ELSD指纹图谱,结合多成分定量分析,比较两种贝母属药材的差异。采用Waters ACQUITY HSS T3(4.6 mm×250 mm,5μm)色谱柱,以乙腈-0.1%三乙胺溶液为流动相,流速为1.1 mL/min,梯度洗脱;柱温为38℃;蒸发光散射检测;建立浙贝母和湖北贝母HPLC-ELSD指纹图谱,通过化学计量学方法和5种生物碱类成分的含量测定比较浙贝母和湖北贝母的差异。结果显示,浙贝母指纹图谱标定7个共有峰,而湖北贝母有8个;指认出其中6个峰,分别为伊贝辛、贝母辛、贝母素甲、贝母素乙、异贝母甲素、湖贝甲素,其中湖贝甲素为湖北贝母的专属性成分;HCA和PCA均能很好地区分浙贝母和湖北贝母,OPLS-DA共找到4个差异性标志物,含测结果显示,浙贝母中贝母素甲的含量明显高于湖北贝母,而贝母辛、贝母素乙和异贝母甲素的含量则明显低于湖北贝母。该方法可以有效鉴别浙贝母和湖北贝母质量的差异性,为其质量控制提供参考。The HPLC-ELSD fingerprints of Fritillariae Thunbergii Bulbus(FTB)and Fritillariae Hupehensis Bulbus(FHB)were established to compare the differences between the two herbs combined with multi-component quantitative analysis.The chromatographic column was Waters ACQUITY HSS T3(4.6 mm×250 mm,5μm).The gradient elution was performed at a flow rate of 1.1 mL/min with acetonitrile-0.1%triethylamine solution as the mobile phase.The column temperature was 38℃.Evaporative light scattering detection was used.The HPLC-ELSD fingerprints of FTB and FHB were established.The differences between FTB and FHB were compared by chemometrics methods and simultaneous determination of five alkaloid components.The results showed that the fingerprints of FTB had seven common peaks and the fingerprints of FHB had eight peaks.Six peaks were identified,namely yibeissine,peimisine,peimine,peiminine,isofritillarine and hupehenine.Among the above ingredients,hupehenine was the specific component of FHB.Both HCA and PCA could distinguish FTB and FHB well.OPLS-DA found four different markers.The content determination results showed that the content of peimine in FTB was significantly higher than that in FHB,while the contents of peimisine,peiminine and isofritillarine were significantly lower than that in FHB.This method can effectively identify the quality differences between FTB and FHB,and provide reference for their quality control.

关 键 词：浙贝母 湖北贝母 HPLC-ELSD指纹图谱 多成分含量测定 

分 类 号：R282.4[医药卫生—中药学]