HPLC-ELSD法同时测定地黄及其不同炮制品中8个糖类成分的含量  

Simultaneous determination of eight saccharides contents in Radix Rehmanniae and its different processed products by HPLC-ELSD

作　　者：薛淑娟 陈越 陈随清[1] 车奇涛 XUE Shu-juan;CHEN Yue;CHEN Sui-qing;CHE Qi-tao(Henan University of Chinese Medicine,Henan Provincial Key Laboratory of Chinese Medicine Resources and Chinese Medicine Chemistry,Zhengzhou 450046,China)

机构地区：[1]河南中医药大学河南省中药资源与中药化学重点实验室,郑州450046

出　　处：《药物分析杂志》2023年第6期939-949,共11页Chinese Journal of Pharmaceutical Analysis

基　　金：国家自然科学基金面上项目(81973477);河南省中医药科学研究专项课题重大专项(2022ZYZD18)。

摘　　要：目的:建立高效液相色谱-蒸发光散射检测器法同时测定地黄及其不同炮制品中8个糖类成分的含量,分析地黄炮制前后糖类成分的含量变化情况。方法:采用XBridge■BEH Amide色谱柱(250 mm×4.6 mm,5μm);30%乙腈水+0.1%氨水(A)和90%乙腈水+0.1%氨水(B)为流动相,梯度洗脱;柱温40℃;漂移管温度105℃;气体流量2.0 L·min^(-1);增益值为1;进样量为10μL。结果:8个糖类成分在各自浓度范围内线性关系良好(r>0.9990),精密度、稳定性、重复性试验结果的RSD均<3.0%,平均加样回收率为97.7%~101.3%。采用主成分分析和方差分析对上述8个糖类成分的含量进行分析,结果显示地黄及其不同炮制品样品中主要含有糖的种类和含量均存在显著差异。结论:该方法简单、准确、重现性好,能有效分析地黄及其不同炮制品中8个糖类成分的含量,为地黄药材的质量控制提供了参考依据。Objective:To establish an high performance liquid chromatography evaporative light scattering detection(HPLC-ELSD)method for simultaneous determination of eight saccharides contents in Radix Rehmanniae(RR)and its different processed products and to analyze the changes of saccharides contents before and after RR processing.Methods:Determination was carried out on a Waters XBridge■BEH Amide column(250 mm×4.6 mm,5μm).The mobile phase was composed of 90% acetonitrile containing 0.1%ammonia(B)and 30% acetonitrile containing 0.1% ammonia(A)with gradient elution.The column temperature was 40℃ and the injection volume was 10μL.Furthermore,the drift tube temperature,carrier gas flow rate and gain value of evaporative light scattering detection were set at 105℃,2.0 L·min~(-1)and 1,respectively.Principal component analysis(PCA)and variance analysis(ANOVA)were used to analyze the contents of the above eight saccharides components.Results:Good separation of 8 components was achieved within 64 min and calibration curves of the eight components showed good linear relationship(r>0.9990).The RSDsof precision,stability and repeatability results were all less than 3.00%,and the average recoveries were within 97.7%-101.3%.PCA and ANOVA results showed that the types and contents of the main saccharides in RR and its different processed products were significantly different.Conclusion:The method is simple,accurate and reproducible,and can effectively analyze the contents of eight saccharides in RR and its different processed products,which provides a reference for the quality control of RR.

关 键 词：地黄 不同炮制品 糖类成分 高效液相色谱-蒸发光散射检测器 含量测定 主成分分析 方差分析 质量控制 

分 类 号：R917[医药卫生—药物分析学]