输血前患者传染病4项检测阳性结果的准确性研究  

Positive accuracy research of four contagious diseases before transfusion

作　　者：袁君 李娜 王伟 范许洲 罗开云 张晓楠 刘倩 栾建凤 YUAN Jun;LI Na;WANG Wei;FAN Xuzhou;LUO Kaiyun;ZHANG Xiaonan;LIU Qian;LUAN Jianfeng(Department of Blood Transfusion Medicine,Eastern Theater Comm and General Hospital,Nanjing,210002,China;Suzhou Xiangcheng District Center for Disease Prevention and Control)

机构地区：[1]东部战区总医院输血医学科,南京210002 [2]苏州市相城区疾病预防控制中心

出　　处：《临床血液学杂志》2023年第6期436-439,444,共5页Journal of Clinical Hematology

基　　金：江苏省卫生厅重大科研项目(No:ZDA2020024)。

摘　　要：目的:探讨东部战区总医院输血医学科传染病4项:乙型肝炎表现抗原(HBsAg)、丙型肝炎抗体(抗-HCV)、人类免疫缺陷病毒抗体(抗-HIV)、梅毒螺旋体抗体(抗-TP),检测结果为阳性的准确性。方法:收集输血医学科酶联免疫吸附试验(ELISA)检测HBsAg阳性标本140例、抗-HCV阳性标本285例、抗HIV阳性标本152例及抗TP阳性标本296例,分别进行中和试验、免疫印迹检测、螺旋体颗粒凝集试验(TPPA)确证其阳性。结果:HBsAg ELISA方法检测阳性标本的阳性确证率为94.3%,抗-HCV阳性确证率为61.8%,抗-HIV阳性确证率为67.1%及抗-TP阳性确证率为76.4%。结论:传染病4项检测S/CO值大于1的阳性结果存在一定假阳性比例,需进一步了解不同区间S/CO值的阳性率及检测确证率,或参考临床肝功能等指标进行判断,为提高检测结果的准确性提供参考和依据。Objective To explore the positive diagnosis rate of four contagious diseases(HBV,HCV,TP,HIV)in department of blood transfusion in Jinling Hospital.Methods We collected 140 HBsAg positive samples,285 anti-HCV positive samples,152 anti-HIV positive samples and 296 anti-TP positive samples by ELISA from the Department of Blood Transfusion Medicine of our hospital,and in order to confirm the diagnosis,neutralization test,Western blot and spirochetes particle agglutination test(TPPA)were used.Results The positive diagnosis rate of HBsAg ELISA was 94.3%,anti-HCV positive diagnosis rate was 61.8%,anti-HIV positive diagnosis rate was 67.1%and anti-TP positive diagnosis rate was 76.4%.Conclusion There was a certain proportion of false positives in the positive results of the four contagious diseases tests with S/CO value greater than 1.It is necessary to further understand the positive rate of S/CO value in different intervals and the detection diagnosis rate,or refer to clinical indicators,such as ALT,AST and CRP values so as to provide reference and basis for improving the accuracy of the detection results.

关 键 词：传染病4项 中和试验 免疫印迹法 螺旋体颗粒凝集试验 阳性确证率 

分 类 号：R446.1[医药卫生—诊断学]