两种动物胆汁酸对热应激小鼠肝脏和空肠保护效果的比较研究  

Comparative Study on the Protective Effects of Two Animal Bile Acids on Liver and Jejunum in Heat-stressed Mice

作　　者：李宁[1] 蒋昀轩 刘德凤 闫国宁 申明月 张树宇 黄博涵 贾宝玉 徐力 魏凯[1] LI Ning;JIANG Yunxuan;LIU Defeng;YAN Guoning;SHEN Mingyue;ZAHNG Shuyu;HUANG Bohan;JIA Baoyu;XU Li;WEI Kai(College of Animal Science and Technology,Shandong Agricultural University,Tai’an 271018,China;Shandong Fanyin Biotechnology Co.,Ltd.,Dongying 257000,China)

机构地区：[1]山东农业大学动物科技学院,泰安271018 [2]山东梵音生物科技有限公司,东营257000

出　　处：《中国畜牧兽医》2023年第7期2729-2739,共11页China Animal Husbandry & Veterinary Medicine

基　　金：山东省现代农业产业技术体系建设经费(SDAIT-10-06)。

摘　　要：【目的】比较猪胆汁酸与羊胆汁酸对热应激小鼠肝脏以及空肠的保护效果。【方法】试验选取40只7周龄BALB/c健康雄性小鼠,随机分为空白对照组(MOCK组)、热应激组(HS组)、热应激-猪胆汁酸组(PBAs组)、热应激-羊胆汁酸组(SBAs组)。试验前7 d,MOCK、HS组小鼠饲喂基础饲粮,PBAs、SBAs组分别在基础饲粮中添加400 mg/kg猪、羊胆汁酸。于试验第8天时,MOCK组小鼠室温放置,其余3组小鼠置于36~38℃、相对湿度60%培养箱中2 h,采集小鼠血液、肝脏和空肠组织,HE染色观察空肠组织病理变化。利用ELISA法检测血清中尿素氮(BUN)含量、碱性磷酸酶(AKP)、谷丙转氨酶(ALT)、谷草转氨酶(AST)活性及肝脏和空肠组织中丙二醛(MDA)、还原型谷胱甘肽(GSH)含量以及超氧化物歧化酶(SOD)活性;采用实时荧光定量PCR检测肝脏和空肠中热休克蛋白60(HSP60)、HSP 70及炎症因子白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)、IL-10基因mRNA表达量。【结果】与MOCK组相比,HS组空肠绒毛高度极显著下降(P<0.01),隐窝深度显著增加(P<0.05);血清ALT活性、肝脏和空肠组织SOD活性均显著降低(P<0.05);肝脏和空肠组织HSP 60、HSP 70基因相对表达量极显著升高(P<0.01)。与HS组相比,PBAs、SBAs组空肠绒毛高度、绒毛高度与隐窝深度比值(V/C)均极显著升高(P<0.01);SBAs组血清ALT活性、空肠组织SOD活性显著或极显著升高(P<0.05;P<0.01);PBAs、SBAs组肝脏组织MDA和GSH含量、HSP 60和HSP 70基因相对表达量均极显著降低(P<0.01),IL-1β、TNF-α基因相对表达量均极显著升高(P<0.01)。另外,SBAs组肝脏、空肠组织HSP 60基因的相对表达量极显著或显著低于PBAs组(P<0.01;P<0.05),肝脏组织IL-1β、TNF-α基因相对表达量显著高于PBAs组(P<0.05)。【结论】在热应激条件下,饲粮中添加羊胆汁酸对于提高小鼠血清ALT活性,提高肝脏和空肠组织抗氧化能力、降低热休克蛋白表达以及修复�【Objective】The purpose of this study was to compare the protective effects of porcine bile acid and sheep bile acid on liver and jejunum of heat stressed mice.【Method】Forty BALB/c healthy male mice aged 7 weeks were randomly divided into blank control group(MOCK group),heat stress group(HS group),heat stress-pig-bile acid group(PBAs group)and heat stress-sheep-bile acid group(SBAs group).Seven days before heat stress,mice in MOCK and HS groups were fed a basal diet,and those in PBAs and SBAs groups were fed the basal diet supplemented with 400 mg/kg pig bile acid and sheep bile acid,respectively.On day 8,mice in MOCK group were placed at room temperature,and mice in other 3 groups were placed in incubators at 36-38℃and 60%relative humidity for 2 h.The blood,liver and jejunum tissues of mice were collected,and the pathological changes of jejunum tissues were observed by HE staining.The content of urea nitrogen(BUN),the activities of alkaline phosphatase(AKP),alanine aminotransferase(ALT)and aspartate aminotransferase(AST)in serum,and the contents of malondialdehyde(MDA),reduced glutathione(GSH)and the activities of superoxide dismutase(SOD)in liver and jejunum tissue were determined by ELISA.The mRNA expression of heat shock protein 60(HSP60),HSP 70 and inflammatory cytokines interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α)and IL-10 genes in liver and jejunum were detected by Real-time quantitative PCR.【Result】Compared with MOCK group,the villus height of jejunum in HS group was extremely significantly decreased(P<0.01),and the crypt depth was significantly increased(P<0.05).The activity of ALT in serum and SOD activity in liver and jejunum were significantly decreased(P<0.05).The relative expressions of HSP 60 and HSP 70 genes in liver and jejunum were extremely significantly increased(P<0.01).Compared with HS group,villus height,and the ratio of villus height and crypt depth(V/C)in jejunum of PBAs and SBAs groups were extremely significantly increased(P<0.01).ALT activity of serum and SOD a

关 键 词：热应激 胆汁酸 损伤 保护效果 

分 类 号：S852.2[农业科学—基础兽医学]