Heterologous production of bioactive xenoacremone analogs in Aspergillus nidulans  

作　　者：LIU Zhiguo LI Wei ZHANG Peng SUN Yi YIN Wen-Bing 

机构地区：[1]State Key Laboratory of Mycology,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China [2]Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China [3]Savaid Medical School,University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Chinese Journal of Natural Medicines》2023年第6期436-442,共7页中国天然药物（英文版）

基　　金：supported by the the National Key Research and Development Program of China(No.2021YFC2300400);the Biological Resources Program,Chinese Academy of Sciences(No.KFJ-BRP-009);the Key Research Program of Frontier Sciences,the Chinese Academy of Sciences(No.ZDBS-LY-SM016).

摘　　要：Tyrosine-decahydrofluorene derivatives are a class of hybrid compounds that integrate the properties of polyketides and nonribosomal peptides.These compounds feature a[6.5.6]tricarbocyclic core and a para-cyclophane ether moiety in their structures and exhibit anti-tumor and anti-microbial activities.In this study,we constructed the biosynthetic pathway of xenoacremones from Xenoacremonium sinensis ML-31 in the Aspergillus nidulans host,resulting in the identification of four novel tyrosine-decahydrofluorene analogs,xenoacremones I–L(1-4),along with two known analogs,xenoacremones A and B.Remarkably,compounds 3 and 4 contained a 12-membered para-cyclophane ring system,which is unprecedented among tyrosine-decahydrofluorene analogs in X.sinensis.The successful reconstruction of the biosynthetic pathway and the discovery of novel analogs demonstrate the utility of heterologous expression strategy for the generation of structurally diverse natural products with potential biological activities.

关 键 词：Heterologous expression Xenoacremones Tyrosine-decahydrofluorene PKS-NRPS Natural products 

分 类 号：Q81[生物学—生物工程]