Single-cell transcriptomic analysis reveals the developmental trajectory and transcriptional regulatory networks of pigment glands in Gossypium bicki  

作　　者：Yue Sun Yifei Han Kuang Sheng Ping Yang Yuefen Cao Huazu Li Qian-Hao Zhu Jinhong Chen Shujin Zhu Tianlun Zhao 

机构地区：[1]College of Agriculture and Biotechnology,Zhejang University,Hangzhou 310058,China [2]Agricultural Experiment Station,Zhejiang University,Hangzhou 310058,China [3]CSIRO Agriculture and Food,GPO Box 1700,Canberra,ACT 2601,Australia [4]Institute of Hainan,Zhejiang University,Hangzhou 310058,China

出　　处：《Molecular Plant》2023年第4期694-708,共15页分子植物（英文版）

基　　金：supported by the Genetically Modified Organisms Breeding Major Project of China(2016ZX08005005);the National Key Technology R&D Program of China(2016YFD0101404);the China Agricultural Research System(CARS-15-27);the Jiangsu Collaborative Innovation Center for Modern Crop Production,and the National Science Foundation of China(32101764).

摘　　要：Comprehensive utilization of cottonseeds is limited by the presence of pigment glands and its inclusion gossypol.The ideal cotton has glandless seeds but a glanded plant,a trait found in only a few Australian wild cotton species,including Gossypium bickii.Introgression of this trait into cultivated species has proved to be difficult.Understanding the biological processes toward pigment gland morphogenesis and the associated underlying molecular mechanisms will facilitate breeding of cultivated cotton varieties with the trait of glandless seeds and glanded plant.In this study,single-cell RNA sequencing(scRNA-seg)was performed on 12222 protoplasts isolated from cotyledons of germinating G.bicki seeds 48 h after imbibition.Clustered into 14 distinct clusters unsupervisedly,these cells could be grouped into eight cell populations with the assistance of known cell marker genes.The pigment gland cells were well separated from others and could be separated into pigment gland parenchyma cells,secretory cells,and apoptotic cells.By integrating the pigment gland cell developmental trajectory,transcription factor regulatory networks,and core transcription factor functional validation,we established a model for pigment gland formation.In this model,light and gibberellin were verified to promote the formation of pigment glands.In addition,three novel genes,GbiERF114(ETHYLENE RESPONSE FACTOR 114),GbiZAT11(ZINC FINGER OF ARABIDOPSIS THALIANA 11),and GbiNTL9(NAC TRANSCRIPTION FACTOR-LIKE 9),Were found to affect pigment gland formation.Collectively,these findings provide new insights into pigment gland morphogenesis and lay the cornerstone for future cotton scRNA-seq investigations.

关 键 词：scRNA-seq pigment gland morphogenesis cell development cotton 

分 类 号：Q94[生物学—植物学]