A chromosome-level genome assembly reveals that tandem-duplicated CYP706V oxidase genes control oridonin biosynthesis in the shoot apex of Isodon rubescens  

作　　者：Yuwei Sun Jie Shao Haili Liu Hua Wang Guangyi Wang Jianhua Li Yaping Mao Zhuo Chen Ke Ma Lin Xu Yong Wang 

机构地区：[1]CAS-Key LaboratoryofSynthetic Bioogy,CAS Center forEcellenceinMolecular Plant Sciences,Insttuteof Plant Physioogy andEoogy,Chinese Academy of Sciences,Shanghai 200032,China [2]University of Chinese Academy of Sciences,Beijing 100039,China [3]National Key Laboratory of Plant Molecular Genetics,CAS Center for Excellence in Molecular Plant Sciences,Institute of Plant Physiology and Ecology,Chinese Academy of Sciences,Shanghai 200032,China

出　　处：《Molecular Plant》2023年第3期517-532,共16页分子植物（英文版）

基　　金：funded by the National Key R&D Program of China(2018YFA0900600);the National Natural Science Foundation of China(32070328,22077129,and 41876084),the Program of Shanghai Academic Research Leader(20XD1404400);the Strategic Priority Research Program“Molecular Mechanism of Plant Growth and Development”of CAS(XDB27020202);the Construction of the Registry and Database of Bioparts for Synthetic Biology of the Chinese Academy of Sciences(ZSYS-016),the International Partnership Program of the Chinese Academy of Sciences(153D31KYSB20170121),Derivative Bank of Chinese Biological Resources(KFJ-BRP-009-002);the National Key Laboratory of Plant Molecular Genetics,SIPPE,CAS.Y.S.is supported by the Foundation of Youth Innovation Promotion Association of the Chinese Academy of Sciences.

摘　　要：The ent-kaurenoids(e.g.,oridonin and enmein)from the Isodon genus(Lamiaceae)are one class of diterpenoids with rich structural diversity and intriguing pharmaceutical activity.In contrast to the well-established gibberellin pathway,oxidative modifications diversifying the ent-kaurene skeleton in Isodon have remained undetermined for half a century.Here we report a chromosome-level genome assembly of I.rubescens,a well-recognized oridonin producer long favored by Asian people as a traditional herb with antitumor effects.The shoot apex was confirmed to be the actual region actively producing ent-kaurene diterpenoids.Through comparative genomics and phylogenetic analyses,we discovered a cluster of tandem-duplicated CYP706V oxygenase-encoding genes located on an ancient genomic block widely distributed in eudicots,whereas almost exclusively emerged in Isodon plants.In the shoot apex,IrCYP706V2 and IrCYP706V7 oxidized the ent-kaurene core in the initial stage of oridonin biosynthesis.Loss of CYP706Vs in other Lamiaceae plants offered an explanation for the specific kaurenoid production in Isodon plants.Moreover,we found that the Isodon genomes encode multiple diterpenoid synthases that are potentially involved in generating diterpenoid diversity.These findings provided new insights into the evolution of the lineage-specific diterpenoid pathway and laid a foundation for improving production of bioactive ent-kaurene-type diterpenoids by molecular breeding and synthetic biology approaches.

关 键 词：oridonin biosynthesis Isodon rubescens genome sequencing CYP706 oxidases diterpenoid diversity 

分 类 号：O62[理学—有机化学]