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作 者:罗霞 谭晓霞 吴三英 陈颖 雷永良 LUO Xia;TAN Xiao-xia;WU San-ying;CHEN Ying;LEI Yong-liang(Lishui Maternal and Child Health Care Hospital,Zhejiang 323000,China)
机构地区:[1]丽水市妇幼保健院,浙江323000
出 处:《中国卫生检验杂志》2023年第12期1513-1515,共3页Chinese Journal of Health Laboratory Technology
基 金:丽水市科技计划项目(2020SJZC016)。
摘 要:目的流感嗜血杆菌是呼吸道常见的条件致病菌,羊膜腔感染是一种常见的妊娠后期并发症。流感嗜血杆菌引起的羊膜腔内感染比较少见,常常导致误诊而耽误治疗。本文通过1例流感嗜血杆菌致羊膜腔内感染的病例资料,建立流感嗜血杆菌体外培养和药敏的方法,为临床流感嗜血杆菌导致的羊膜腔感染的诊治提供帮助。方法收集1例羊膜腔内感染孕妇的痰液、血液、羊水,进行细菌平板培养,以V、X、V+X因子需求试验鉴定流感嗜血杆菌,并进行药敏实验。结果通过巧克力平板培养法,在痰液、血液、羊水,均成功培养出流感嗜血杆菌,并成功鉴定,药敏结果显示对头孢噻肟敏感。结论通过体外细菌培养,并结合药敏实验,能够有效诊断和治疗流感嗜血杆菌致羊膜腔内感染。Objective Haemophilus influenzae is a common opportunistic pathogen in the respiratory tract,and amniotic cavity infection is a common late pregnancy complication.Amniotic cavity infection caused by Haemophilus influenzae is relatively rare,often leading to misdiagnosis and delayed treatment.In this paper,based on the data of a case of amniotic cavity infection caused by Haemophilus influenzae,the method of in vitro culture and drug sensitivity of Haemophilus influenzae was established to provide help for the diagnosis and treatment of similar clinical cases.Methods Sputum,blood and amniotic fluid of a pregnant woman with intra-amniotic infection were collected.Bacterial culture was performed by plate culture method.And factor V,X and V+X requirement tests were used to identify Haemophilus influenzae,and drug sensitivity tests were performed.Results Haemophilus influenzae in sputum,blood,amniotic fluid was successfully cultured and identified on chocolate plates,and drug susceptibility results showed that it was susceptible to cefotaxime.Conclusion It is an effective method to treat intra-amniotic infection caused by Haemophilus influenzae by in vitro bacterial culture and selection of sensitive drugs.
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