39428例抗着丝点抗体检测结果分析  

Detection and analysis of anti-centromere antibodies in 39428 cases

作　　者：周思思 张盟 陶洪群[1] ZHOU Si-si;ZHANG Meng;TAO Hong-qun(Clinical Laboratory Center,the Second Affiliated Hospital of Wenzhou Medical University,Zhejiang 325000,China)

机构地区：[1]温州医科大学附属第二医院临床检验中心,浙江325000

出　　处：《中国卫生检验杂志》2023年第13期1629-1632,共4页Chinese Journal of Health Laboratory Technology

摘　　要：目的分析不同疾病中抗着丝点抗体(ACA)的阳性分布情况。方法收集39428例不同疾病检测ACA抗体的患者资料,回顾性分析ACA分布情况。ACA采用间接免疫荧光法和多重微珠流式免疫荧光法检测。结果不同疾病中ACA的阳性率不同,原发性胆汁性肝硬化的阳性率最高(33.33%),其次为雷诺现象(16.82%),干燥综合征的阳性率为7.32%,自身免疫性肝炎的阳性率为6.52%,混合性结缔组织病的阳性率为5.84%,系统性硬化症的阳性率为6.11%,强直性脊柱炎的阳性率为3.49%,类风湿性关节炎的阳性率为3.60%。在649例ACA阳性(任一种方法结果为阳性)患者中,有272例2种方法检测均为阳性,有377例其中一种方法检测阳性;647例间接免疫荧光法阳性的患者中,1:1000有191例,1:100有104例,1:320有85例,1:3200有38例,1:10000有8例。结论ACA可在多种疾病中存在,自身免疫性疾病ACA阳性率高于非自身免疫性疾病;自身免疫性疾病2种方法检测ACA同时阳性的概率大于非自身免疫性疾病;自身免疫性疾病患者ACA荧光滴度以中高滴度为主,非自身免疫性疾病患者ACA荧光滴度以低滴度为主;ACA阳性患者血清中可出现其他多种自身抗体;当患者出现ACA阳性时,需进一步结合其他检测指标和临床症状进行综合判断。Objective This paper aims to analyze the positive distribution of anti-centromere antibody(ACA)in different dis⁃eases.Methods The data of 39428 patients with ACA antibody in different diseases were collected and the distribution of ACA was analyzed retrospectively.ACA was detected by indirect immunofluorescence and multiple bead flow immunofluores⁃cence.Results The positive rate of ACA was different in different diseases.The positive rate of primary biliary cirrhosis was the highest(33.33%),followed by Raynaud's phenomenon(16.82%),Sjogren's syndrome(7.32%),autoimmune hepatitis(6.52%),mixed connective tissue disease(5.84%),systemic sclerosis(6.11%),ankylosing spondylitis(3.49%),rheu⁃matoid arthritis(3.60%).Among the 649 patients with ACA positive(the results of either method were positive),272 were positive by both methods,and 377 were positive by one of them.Among the 647 patients with indirect immunofluorescence,191 cases were 1:1000,104 were 1:100,85 were 1:320,38 were 1:3200,and 8 were 1:10000.Conclusion ACA can exist in many diseases,and the positive rate of ACA in autoimmune diseases is higher than that in non-autoimmune diseases.The positive rate of ACA detected by two methods in autoimmune diseases is higher than that in non-autoimmune diseases.The fluorescence titer of ACA in patients with autoimmune diseases was mainly medium and high titer,while that in patients with non-autoimmune diseases was mainly low titer.There are many other autoantibodies in the serum of ACA positive patients.When the patient is ACA positive,it needs to be further combined with other detection indicators and clinical symptoms for comprehensive judgment.

关 键 词：抗着丝点抗体 自身抗体 间接免疫荧光法 多重微珠流式免疫荧光法 

分 类 号：R44[医药卫生—诊断学]