皱叶重楼根茎的化学成分及抗菌活性研究  

Chemical constituents from Paris rugosa rhizomes and their antimicrobial activities

作　　者：段晓燕 岳美岑 杨珺[1] 白雪[1] 罗吉凤 李恒[1] 王跃虎 DUAN Xiao-yan;YUE Mei-cen;YANG Jun;BAI Xue;LUO Ji-feng;LI Heng;WANG Yue-hu(Key Laboratory of Plant Resources and Biotechnology,Yunnan Key Laboratory for Wild Plant Resources,State Key Laboratory of Phytochemistry and Plant Resources in West China,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650201,China)

机构地区：[1]中国科学院、昆明植物研究所、资源植物与生物技术重点实验室、云南省野生资源植物研发重点实验室、植物化学与西部植物资源持续利用国家重点实验室,云南昆明650201

出　　处：《中国中药杂志》2023年第11期2981-2988,共8页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(32070361)。

摘　　要：藜芦科植物皱叶重楼目前仅分布于我国云南省,其化学成分尚未被系统地研究。该研究通过多种柱色谱方法及半制备高效液相等分离手段,从皱叶重楼根茎的乙醇提取物中分离鉴定了9个化合物,分别为pariposide G(1)、cerin(2)、豆甾-4-烯-3-酮(3)、β-蜕皮激素(4)、麦冬皂苷C′(5)、甲基原纤细薯蓣皂苷(6)、纤细薯蓣皂苷(7)、重楼皂苷H(8)、parisyunnanoside G(9)。其中化合物1为新化合物,化合物1~9均为首次从该植物中分离得到。对所有化合物的抗细菌和真菌活性进行了评价,实验结果表明麦冬皂苷C′对白色念珠菌[MIC90=(4.68±0.01)μmol·L^(-1)]和白色念珠菌氟康唑耐药株[MIC90=(4.66±0.02)μmol·L^(-1)]有较强的抑制作用。Paris rugosa(Melanthiaceae)only grows in Yunnan province of China at present,and its chemical constituents have not been systematically studied.In this study,nine compounds,including one new compound pariposide G(1)and eight known compounds of cerin(2),stigmast-4-en-3-one(3),β-ecdysone(4),ophiopogonin C′(5),methyl protogracillin(6),gracillin(7),parissaponin H(8),and parisyunnanoside G(9),were isolated and identified from the ethanol extract of P.rugosa rhizomes by column chromatography methods and semi-preparative high-performance liquid chromatography(HPLC).Compounds 1-9 were isolated from this plant for the first time.The antibacterial and antifungal activities of all the compounds were evaluated.The results showed that ophiopogonin C′had strong inhibitory effects on Candida albicans[MIC90=(4.68±0.01)μmol·L^(-1)]and the fluconazole-resistant strain of C.albicans[MIC90=(4.66±0.02)μmol·L^(-1)].

关 键 词：藜芦科 皱叶重楼 甾体皂苷 抗菌活性 白色念珠菌氟康唑耐药株 

分 类 号：R284.1[医药卫生—中药学] R285[医药卫生—中医学]