基于UPLC指纹图谱及多成分含量测定的火草质量控制研究  

Quality evaluation of Huocao based on UPLC fingerprint and multi-component content determination

作　　者：杨正明 地久此呷 兰建龙 罗江 海来约布 王涛 李文兵 李莹 刘圆 YANG Zheng-ming;DIJIU Ci-ga;LAN Jian-long;LUO Jiang;HAILAI Yue-bu;WANG Tao;LI Wen-bing;LI Ying;LIU Yuan(Institute of Qinghai-Tibetan Plateau,Southwest Minzu University,Chengdu 610225,China;College of Pharmacy,Southwest Minzu University,Chengdu 610225,China;Sichuan Provincial Qiang-Yi Medicinal Resources Protection and Utilization Technology Engineering Laboratory,Chengdu 610225,China;Tibetan Plateau Ethnic Medicinal Resources Protection and Utilization Key Laboratory of National Ethnic Affairs Commission of the People′s Republic of China,Chengdu 610225,China;Ethnic Medicine Institute,Southwest Minzu University,Chengdu 610225,China)

机构地区：[1]西南民族大学青藏高原研究院,四川成都610225 [2]西南民族大学药学院,四川成都610225 [3]四川省羌彝药用资源保护与利用技术工程实验室,四川成都610225 [4]青藏高原民族药用资源保护与利用国家民委重点实验室,四川成都610225 [5]西南民族大学民族医药研究院,四川成都610225

出　　处：《中国中药杂志》2023年第11期3000-3013,共14页China Journal of Chinese Materia Medica

基　　金：国家重点研发计划项目(2018YFC1708005);四川省科技计划项目(2021YFS0043);中央高校基本科研业务费专项资金研究类项目(ZD2022940)。

摘　　要：火草灸是彝族医用于治疗寒湿性病症的特色适宜技术,火草作为火草灸的施灸材料,临床用药混乱,无质量控制相关研究。该文针对火草的非挥发性成分,采用UPLC建立火草非挥发性成分化学指纹图谱及绿原酸等8个酚酸类成分的含量测定方法,采用多元统计分析方法寻找火草药材质量评价指标性成分,建立火草质量的综合评价体系。建立了49批火草药材UPLC指纹图谱,标定了20个共有峰,指认了新绿原酸、绿原酸等8个酚酸类成分。除3批药材外,火草药材的相似度均高于0.89,表明所建立的指纹图谱方法可用于火草药材的整体质量控制;火草中8个成分含量熵权得分与指纹图谱综合得分的相关系数为0.875(P<0.01),表明所测的8个成分可作为指标性成分对火草进行质量评价。进一步,结合指纹图谱共有峰与8个酚酸类成分的含量,进行多元统计分析,筛选得到绿原酸、异绿原酸A、异绿原酸C为火草质量评价指标。结果表明,所建立的方法简便、准确,可实现基于UPLC指纹图谱和多成分含量测定的火草药材质量控制,该结果可为火草的质量标准建立提供数据支撑。Huocao(a traditional Chinese herbal medicine)moxibustion is a characteristic technology in Yi medicine suitable for cold-dampness diseases.Huocao,as the moxibustion material,is confusedly used in clinical practice and little is known about its quality control.In this study,UPLC method was used to establish the chemical fingerprint of non-volatile components in Huocao,and the contents of eight phenolic acids such as chlorogenic acid were determined.Multivariate statistical analysis was performed to obtain the indicator components of Huocao for quality evaluation,and thus a comprehensive evaluation system for the quality of Huocao was built.The UPLC fingerprints of 49 batches of Huocao were established,and there were 20 common peaks,of which eight phenolic acids including neochlorogenic acid and chlorogenic acid were identified.Except for three batches of Huocao,the similarity of the other 46 batches was higher than 0.89,suggesting that the established fingerprint method could be used for quality control of the medicinal herb.The correlation coefficient between entropy weight score of the eight phenolic acids and comprehensive fingerprint score in Huocao was 0.875(P<0.01),which indicated that the eight phenolic acids could be used as indicator components for the quality evaluation of Huocao.Furthermore,in multivariate statistical analysis on the common peaks of fingerprint and the contents of the eight phenolic acids,chlorogenic acid,isochlorogenic acid A and isochlorogenic acid C were screened to be the indicator components.The results revealed that the proposed method achieved a simple and accurate quality control of Huocao based on UPLC fingerprint and multicomponent content determination,which provided useful data for establishing the quality standard of Huocao.

关 键 词：火草 川西火绒草 松毛火绒草 珠光香青 指纹图谱 含量测定 多元统计分析 

分 类 号：R284[医药卫生—中药学] O657.72[医药卫生—中医学]