德州驴饲粮中营养物质表观消化率不同测定方法的比较  

Comparison of Different Methods for Determining Apparent Digestibility of Nutrients in Diets of Dezhou Donkeys

作　　者：杨泉 王云[1] 张晨 张桂国 张崇玉 YANG Quan;WANG Yun;ZHANG Chen;ZHANG Guiguo;ZHANG Chongyu(Key Laboratory of Efficient Utilization of Non-Grain Feed Resources of the Ministry of Agriculture and Rural Affairs(Jointly Built by the Ministry and the Province),Shandong Provincial Key Laboratory of Animal Bioengineering and Disease Prevention,School of Animal Science and Technology,Shandong Agricultural University,Tai’an 271018,China)

机构地区：[1]山东农业大学动物科技学院,山东省动物生物工程与疾病防治重点实验室,农业农村部非粮饲料资源高效利用重点实验室(部省共建),泰安271018

出　　处：《动物营养学报》2023年第7期4465-4474,共10页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：山东省现代农业产业技术体系(SDAIT⁃27⁃03,SDAIT⁃23⁃05);驴良种高效繁育及配套饲养技术集成与示范(S190503110001⁃1cy)。

摘　　要：本试验旨在对德州驴饲粮中营养物质表观消化率的不同测定方法进行比较。试验选用体重(128.5±5.0)kg左右的9月龄德州驴公驴12头,随机分为3个处理(每个处理4头驴),分别饲喂单一饲草苜蓿颗粒、含60%粗料的高粗料全混合日粮颗粒(TMR-1)和含30%粗料的低粗料全混合日粮颗粒(TMR-2)这3种颗粒型饲粮,每种饲粮独立地利用全收粪法、酸性洗涤木质素(ADL)法和盐酸不溶灰分(AIA)法进行消化试验。消化试验共16 d,其中预试期10 d,正试期6 d。德州驴饲养在单体代谢笼内,全收粪法在正试期内24 h值班,粪便随排随收,于每天08:00准确记录排粪量和采食量;ADL法与AIA法在正试期内每天取3次新鲜粪样,每次约100 g。利用ADL法、AIA法和全收粪法分别测定德州驴饲粮中营养物质的表观消化率。结果显示:1)测定相同饲粮时,ADL法测得的消化能(DE)以及干物质(DM)、有机物(OM)、粗蛋白质(CP)、粗脂肪(EE)、粗纤维(CF)、无氮浸出物(NFE)、碳水化合物(CHO)、中性洗涤纤维(NDF)、酸性洗涤纤维(ADF)、半纤维素(HCEL)和纤维素(CEL)的表观消化率与全收粪法基本一致,均差异不显著(P>0.05);AIA法测得的DE以及DM、OM、CP、EE、CF、NFE、CHO、NDF、ADF、HCEL和CEL的表观消化率则显著高于全收粪法(P<0.05)。2)采用相同方法(ADL法)测定德州驴对不同粗料水平饲粮的营养物质表观消化率时,TMR-2的DE以及DM、OM、CP、EE、NFE和CHO的表观消化率显著高于TMR-1和苜蓿颗粒(P<0.05)。综上所述,ADL可以作为理想的内源指示剂测定德州驴饲粮中营养物质的表观消化率;随着颗粒型饲粮中粗料水平的升高,德州驴对饲粮中DE以及DM、OM、CP、EE、NFE和CHO的表观消化率逐渐降低。The purpose of this experiment was to compare the different methods for determining apparent di⁃gestibility of nutrients in the diet of Dezhou donkeys.In the experiment,twelve 9⁃month⁃old Dezhou donkeys weighing about(128.5±5.0)kg were randomly divided into 3 treatments with 4 donkeys in each treatment.Three kinds of granular diets were fed separately,namely,single forage alfalfa granules,high roughage total mixed granular diet(TMR⁃1)containing 60%roughage and low roughage total mixed granular diet(TMR⁃2)containing 30%roughage.Each diet was independently digested by total fecal collection method,acid detergent lignin(ADL)method and hydrochloric acid insoluble ash(AIA)method.The digestion test lasted for 16 days,including 10 days for the preliminary test and 6 days for the formal test.The Dezhou donkey was raised in a single metabolic cage.The full fecal collection method was on duty 24 hours during the formal test.The feces were collected as they were discharged.The fecal excretion and feed intake were accurately recorded at 08:00 every day;during the formal test,ADL method and AIA method took fresh fecal samples three times a day,about 100 g each time.The ADL method,AIA method and total fecal collection method were used to determine the apparent digestibility of nutrients in the diet of Dezhou donkeys.The results showed as follows:1)the digestible energy(DE)and the apparent digestibility of dry matter(DM),organic matter(OM),crude protein(CP),ether extract(EE),crude fiber(CF),nitrogen⁃free extract(NFE),carbohydrate(CHO),neutral detergent fiber(NDF),acid detergent fiber(ADF),hemicellulose(HCEL)and cellulose(CEL)in the same diet determined by the total fecal collection method and ADL method were basically the same,the differences were not significant(P>0.05);the DE and the apparent digestibility of DM,OM,CP,EE,CF,NFE,CHO,NDF,ADF,HCEL and CEL in the same diet determined by AIA method were signifi⁃cantly higher than those determined by total fecal collection method(P<0.05).2)When the same method(ADL me

关 键 词：全收粪法 ADL法 AIA法 德州驴 消化率 

分 类 号：S822[农业科学—畜牧学]