敲除tp53对斑马鱼高温耐受能力和运动能力的影响  

Effect of tp53 Knockout on High Temperature Tolerance and Swimming Capacity in Zebrafish

作　　者：杨倩婷 田晓英 张俊芳 韩兵社 YANG Qianting;TIAN Xiaoying;ZHANG Junfang;HAN Bingshe(National Demonstration Center for Experimental Fisheries Science Education,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Ministry of Education,College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海海洋大学水产与生命学院,水产种质资源发掘与利用教育部重点实验室,水产科学国家级实验教学示范中心,上海201306

出　　处：《生物技术进展》2023年第4期580-587,共8页Current Biotechnology

基　　金：国家自然科学基金项目(81770165);上海市科技兴农重点攻关项目[沪农科创字〔2019〕第1-2号];上海市教委水产一流学科建设项目。

摘　　要：研究了敲除tp53基因对斑马鱼高温耐受能力和运动能力的影响。通过RT-qPCR、Western blot等技术发现高温下野生型的斑马鱼tp53表达上调,暗示tp53可能在高温下发挥作用,而敲除tp53基因的斑马鱼在高温下的存活时间降低。进一步研究发现,tp53-/-斑马鱼在高温下ROS水平升高(P<0.05),ATP水平降低(P<0.001),γH2A.X蛋白水平显著升高。通过苏木精-伊红(hematoxylin-eosin,HE)染色发现tp53-/-斑马鱼高温下肝脏组织充血更严重。Danio Vision斑马鱼行为轨迹分析显示34℃下tp53-/-斑马鱼的运动能力减弱。研究结果表明,tp53在高温下对斑马鱼的高温耐受能力和运动能力起正向调节作用,为斑马鱼高温耐受机制和运动行为学研究提供了新思路。The aim of the study was to investigate the effect of tp53 knockout on high temperature tolerance and swimming capacity in zebrafish.Through RT-qPCR,Western blot and other technologies,it was found that the expression of tp53 in wild-type zebrafish at high temperature was upregulated,suggesting that tp53 may play a role at high temperature,while zebrafish with the tp53 gene knockout had a reduced survival time at high temperature.Further studies found that tp53-/-zebrafish had increased ROS levels(P<0.05),decreased ATP levels(P<0.001),and significantly increased levels ofγH2A.X protein at high temperatures.It was found by hematoxylin-eosin(HE)staining that the liver tissue hyperemia of tp53-/-zebrafish was more severe at high temperatures.Danio Vision behavior trajectory analysis showed that tp53-/-zebrafish exercise capacity was weakened at 34℃.The results showed that tp53 has a positive effect on the high temperature tolerance and swimming capacity of zebrafish,which provided a new idea for the study of high temperature tolerance mechanism and exercise behavior in zebrafish.

关 键 词：斑马鱼 高温 TP53 运动能力 肝 

分 类 号：Q958.8[生物学—动物学]