番茄黄化曲叶病毒安徽分离物的基因组结构特征及变异分析  

Genomic structure characteristics and variation analysis of tomato yellow leaf curl virus Anhui isolate

作　　者：蒋磊[1,2,3] 徐世强 徐凯 丁菲 江彤[1,2,3] JIANG Lei;XU Shiqiang;XU Kai;DING Fei;JIANG Tong(School of Plant Protection,Anhui Agricultural University,Hefei 230036;Anhui Province Key Laboratory of Integrated Pest Management on Crops,Hefei 230036;Key Laboratory of Biology and Sustainable Management of Plant Diseases and Pests of Anhui Higher Education Institutes,Hefei 230036)

机构地区：[1]安徽农业大学植物保护学院,合肥230036 [2]作物有害生物综合治理安徽省重点实验室,合肥230036 [3]植物病虫害生物学与绿色防控安徽省普通高校重点实验室,合肥230036

出　　处：《安徽农业大学学报》2023年第3期446-449,共4页Journal of Anhui Agricultural University

基　　金：安徽省重点研究与开发计划项目(202004a06020013);大学生创新创业训练计划项目(dg193404);安徽省博士后基金项目(2019B360)共同资助

摘　　要：利用滚环扩增(RCA)技术从安徽淮北番茄温室表现曲叶症状的番茄上获得番茄黄化曲叶病毒(tomato yellow leaf curl virus,TYLCV)的2个分离物全基因组。2个TYLCV DNA-A核苷酸序列全长均为2781 nts,共编码6个ORF。序列比对结果表明,2个TYLCV安徽分离物DNA-A核苷酸序列同源性为99.7%,与已报道的国内外其他24个TYLCV各分离物同源性高达97.8%~99.9%。系统进化分析显示,TYLCV安徽分离物与来自吉林、沈阳、天津、邯郸和山东的5个TYLCV分离物亲缘关系最近。The whole genomes of two isolates of tomato yellow leaf curl virus(TYLCV)from the tomatos exhibited curved leaf symptom in greenhouse of Huaibei,Anhui Province were obtained by rolling circle amplifi-cation(RCA).The two nucleic acid sequences of TYLCV DNA-A were both 2781 nucleotides(nts)in length,containing six potential ORFs.The results of sequence alignment showed that the nucleic acid sequence homology of the two TYLCV Anhui isolates was 99.7%,and they shared identity of 97.8%-99.9%with other 24 TYLCV isolates in domestic and foreign.Phylogenetic analysis showed that TYLCV Anhui isolate had the closest rela-tionships with the other five TYLCV isolates from Jilin,Shenyang,Tianjin,Handan and Shandong.

关 键 词：滚环扩增 番茄黄化曲叶病毒 序列分析 

分 类 号：S432.41[农业科学—植物病理学]