Fabrication of glycidyl methacrylate-modified silk fibroin/poly(L-lactic acid-co-ε-caprolactone)-polyethylene glycol diacrylate hybrid 3D nanofibrous scaffolds for tissue engineering  

作　　者：Yongyong Fan Anlin Yin Yunhuan Li Qi Gu Yan Zhou Junlong Zhou Ruibo Zhao Kuihua Zhang 

机构地区：[1]Institute of Smart Biomaterials,School of Materials Science and Engineering and Zhejiang-Mauritius Joint Research Center for Biomaterials and Tissue Engineering,Zhejiang Sci-Tech University,Hangzhou 310018,China [2]College of Materials and Textile Engineering,Nanotechnology Research Institute,Jiaxing University,Jiaxing 314001,China

出　　处：《Frontiers of Materials Science》2023年第2期79-91,共13页材料学前沿（英文版）

基　　金：This work was sponsored by the Jiaxing Public Welfare Research Project(Grant Nos.2021AY10062 and 2021AY10063);the Zhejiang Provincial Natural Science Foundation of China(Grant Nos.LY20C100003 and LY22C100001);the National Undergraduate Training Program for Innovation and Entrepreneurship(Grant No.202010354009).

摘　　要：In order to provide a biomimetic natural extracellular matrix microenvironment with excellent mechanical capacity for tissue regeneration,a novel porous hybrid glycidyl methacrylate-modified silk fibroin/poly(L-lactic acid-co-ε-caprolactone)–polyethylene glycol diacrylate(SFMA/P(LLA-CL)–PEGDA)hybrid three-dimensional(3D)nanofibrous scaffolds was successfully fabricated through the combination of 3D nanofibrous platforms and divinyl PEGDA based photocrosslinking,and then further improved water resistance by ethanol vapor post-treatment.Scanning electron microscopy and micro-computed tomography results demonstrated significant PEGDA hydrogel-like matrices bonded nanofibers,which formed a 3D structure similar to that of“steel bar(nanofibers)‒cement(PEGDA)”,with proper pore size,high porosity,and high pore connectivity density.Meanwhile,the hybrid 3D nanofibrous scaffolds showed outstanding swelling properties as well as improved compressive and tensile properties.Furthermore,these hybrid 3D nanofibrous scaffolds could provide a biocompatible microenvironment,capable of inducing the material‒cell hybrid and regulating human umbilical vein endothelial cells proliferation.They thus present significant potential in tissue regeneration.

关 键 词：hybrid 3D nanofibrous scaffold silk fibroin tissue engineering human umbilical vein endothelial cell 

分 类 号：R318.08[医药卫生—生物医学工程]