广西壮族自治区玉林市健康人携带单相鼠伤寒沙门菌耐药及分子遗传特征研究  

Antimicrobial resistance and molecular genetic characteristics of monophasic Salmonella Typhimurium carried by healthy population in Yulin,Guangxi Zhuang Autonomous Region

作　　者：罗铭[1] 王鲁彦 周昶宇 侯心娇 凌誌穗 温渝健 莫宗鑫 夏樱花 吴炎农 闫梅英[2] Luo Ming;Wang Luyan;Zhou Changyu;Hou Xinjiao;Ling Zhisui;Wen Yujian;Mo Zongxin;Xia Yinghua;Wu Yannong;Yan Meiying(Yulin Prefectural Center for Disease Control and Prevention,Yulin 537000,Guangxi,China;State Key Laboratory for Communicable Disease Prevention and Control,National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China)

机构地区：[1]玉林市疾病预防控制中心,广西玉林537099 [2]中国疾病预防控制中心传染病预防控制所,传染病预防控制国家重点实验室,北京102206

出　　处：《疾病监测》2023年第6期714-721,共8页Disease Surveillance

基　　金：传染病预防控制国家重点实验室开放课题(No.2022SKLID303)。

摘　　要：目的了解广西壮族自治区玉林市健康人携带的单相鼠伤寒沙门菌的耐药情况及菌株间的同源性。方法对2021—2022年分离自健康饮食从业人群的147株单相鼠伤寒沙门菌进行血清学鉴定,采用微量肉汤稀释法进行16种药物的敏感性试验,应用脉冲场凝胶电泳(PFGE)进行分子分型及聚类分析,同时进行全基因组测序及多位点序列分型分析。结果单相鼠伤寒沙门菌普遍耐药,耐药菌株占98.64%,对链霉素的耐药率最高(96.60%),其次为氨苄西林和四环素(均为91.16%)。多重耐药率高达85.71%,主要耐药谱为氨苄西林–链霉素–四环素三重耐药。147株菌分为132种PFGE带型,研究发现成簇菌株存在,但菌株基因组遗传差异较大。核心基因组进化树分析提示存在3起聚集。多位点序列分型显示,序列型ST34占绝对优势(96.60%)。结论玉林市健康人携带单相鼠伤寒沙门菌耐药严重,菌株具有明显的遗传多样性,存在潜在暴发风险,提示应加强重点人群健康监测及暴露危险因素的调查,降低该耐药新血清型沙门菌扩散风险。Objective To understand the drug resistance and phylogenetic characteristics of monophasic Salmonella Typhimurium isolated from healthy population in Yulin,Guangxi Zhuang autonomous region.Methods A total of 147 strains of monophasic S.Typhimurium were isolated from healthy people engaged in food process from 2021 to 2022 for serotyping and drug susceptibility test to 16 antimicrobial agents using micro-broth dilution method.Molecular subtyping and cluster analysis were performed by pulse-field gel electrophoresis(PFGE).Whole genome sequencing and multi locus sequence typing were performed to explore the genetic characteristics of the strains.Results The drug resistance of monophasic S.Typhimurium was common.The drug resistant strains accounted for 98.64%.The resistance of monophasic S.Typhimurium to streptomycin was highest(96.60%),followed by ampicillin(91.16%)and tetracycline(91.16%).The multidrug resistant rate was 85.71%,and the main drug resistant profile was resistant to ampicillin,streptomycin and tetracycline simultaneously.A total of 132 PFGE patterns were identified in 147 strains,and some clusters were observed,but the strains in the clusters showed high genetic diversities in genomes.The phylogenetic tree based on core genome showed three potential clonal clusters in 147 strains.Multi locus sequence typing showed that ST34 was the predominant type,accounting for 96.60%of all strains.Conclusion The drug resistance of monophasic S.Typhimurium carried by healthy population in Yulin was serious.The strains had high genetic diversity,indicating the potential for outbreaks.The study results suggest that health monitoring and risk factor identification should be further strengthened to reduce the spread of emerging serotype of Salmonella with high antimicrobial resistance.

关 键 词：单相鼠伤寒沙门菌 健康人 耐药 分子遗传 

分 类 号：R211[医药卫生—中医学] R378.22