贯叶连翘提取物对慢性复杂应激小鼠的抗抑郁、焦虑作用研究  

Antidepressant and anxiolytic effects of St.John′s wort extract on chronic complex stress mice

作　　者：朱雪锋 杜丽 程晓晨 卢育新 贺冬秀[1] 赖成材 高月 张庆林[2] ZHU Xue-feng;DU Li;CHENG Xiao-chen;LU Yu-xin;HE Dong-xiu;LAI Cheng-cai;GAO Yue;ZHANG Qing-lin(Institute of Pharmacy&Pharmacology,University of South China,Hengyang,Hunan 421001,China;Institute of Radiation Medicine,Academy of Military Medical Sciences,Academy of Military Sciences,Beijing 100850,China)

机构地区：[1]南华大学药物药理研究所,湖南衡阳421001 [2]军事科学院军事医学研究院辐射医学研究所,北京100850

出　　处：《军事医学》2023年第6期454-459,共6页Military Medical Sciences

摘　　要：目的构建模拟复杂作业环境的慢性复杂应激小鼠模型,并研究贯叶连翘提取物对慢性复杂应激小鼠的抗抑郁、焦虑作用。方法将48只小鼠随机分为6组,即空白对照组,模型组,盐酸舍曲林组,贯叶连翘提取物(St.John's wort extract,SJW⁃E)高、中、低剂量组,每组8只。除空白对照组外,其他组均经历慢性复杂应激过程:小鼠置于带有气孔的离心管中束缚,并于低速振荡器中晃动,同时暴露于噪声和非自然光照中,以上过程6 h/d,持续8周。空白对照组和模型组给予生理盐水,其余组分别给予盐酸舍曲林(15 mg/kg)、贯叶连翘提取物(25、50、100 mg/kg)。8周后,采用旷场实验、悬尾实验、高架十字迷宫实验、强迫游泳实验、Morris水迷宫实验检测小鼠行为学变化;采用HE染色观察小鼠脑海马组织的病理变化。结果8周后,相比于模型组,贯叶连翘提取物组小鼠悬尾不动时间和游泳不动时间明显缩短,旷场中央区域停留时间和路程明显增加,高架十字迷宫中的开臂停留时间和进入次数明显增加,海马神经元损伤得到明显改善,且效果与阳性对照药物舍曲林效果相当。结论贯叶连翘提取物能够显著改善慢性复杂应激小鼠的焦虑和抑郁症状。Objective To construct chronic complex stress mouse models that simulate complex operation environments and investigate the antidepressant and anxiolytic effects of St.John's wort extract(SJW⁃E)on chronic complex stress mice.Methods The mice were randomly divided into 6 groups with 8 animals in each:control group,model group,sertraline⁃treated group(15 mg/kg),and SJW⁃E⁃treated groups(25,50,100 mg/kg).Except for the mice in the control group,all the other mice were subjected to chronic complex stress for 6 hours a day for 8 weeks.Firstly,mice were individually placed into a well⁃ventilated centrifuge tube,Next,the mice in bondage were placed in an oscillator with low⁃speed vibration.Meanwhile,they were exposed to noise and unnatural light.The mice in the control and the model group were intragastrically administered with saline,while the treatment groups with SJW⁃E or sertraline.Behavioral changes were tested by the tail suspension test(TST),forced swimming test(FST),open field test(OFT),elevated plus maze tests(EPMT)and Morris water maze test(WMT)after 8 weeks.Pathological changes in hippocampal tissues of the brain of mice were observed using hematoxylin and eosin(H&E)staining.Results Compared with the model group,the immobility time of the mice in the SJW⁃E groups significantly decreased in the TST and the FST,while the time spent and traveled distance in the central square of the OFT and the time spent and number of entries in the open arms of the EPMT in the SJW⁃E groups were increase.The damage to hippocampal neurons was significantly improved after 8 weeks.Conclusions SJW⁃E can improve depression and anxiety symptoms induced by chronic complex stress.

关 键 词：贯叶连翘提取物 应激 小鼠 抑郁 焦虑 

分 类 号：R285.5[医药卫生—中药学] R749.72[医药卫生—中医学]