老年男性高血压性左心室肥厚患者循环微RNA差异表达分析  

Differential Expression of Circulating MicroRNA in Elderly Male Patients with Hypertensive Left Ventricular Hypertrophy

作　　者：刘子凡 郝本川 钟国徽 厉建伟 杜锐凯 凌树宽[3] 张然[1,2] LIU Zifan;HAO Benchuan;ZHONG Guohui;LI Jianwei;DU Ruikai;LING Shukuan;ZHANG Ran(Department of Cardiovascular Medicine,Chinese PLA General Hospital,Beijing 100853,China;Chinese PLA Medical School,Beijing 100853,China;State Key Laboratory of Space Medicine Fundamentals and Application,China Astronaut Research and Training Center,Beijing 100094,China)

机构地区：[1]中国人民解放军总医院心血管病医学部,北京市100853 [2]中国人民解放军医学院,北京市100853 [3]中国航天员科研训练中心航天医学基础与应用国家重点实验室,北京市100094

出　　处：《中国分子心脏病学杂志》2023年第3期5353-5361,共9页Molecular Cardiology of China

基　　金：航天医学基础与应用国家重点实验室研究基金(SMFA19A02)。

摘　　要：目的分析老年男性高血压性左心室肥厚(LVH)患者血浆微RNA(miRNA)表达水平,探究与高血压发生及进展为高血压性LVH相关的miRNA。方法选择2021年1月至2022年11月在中国人民解放军总医院就诊的老年男性83例,其中43例为高血压伴LVH(高血压合并LVH组),40例为高血压不伴LVH(高血压组);选取同期体检的43名健康老年男性作为健康对照组。首先选取高血压合并LVH组及健康对照组各3例进行miRNA高通量测序,应用实时荧光定量PCR(qRT-PCR)分别在测序样本及大样本中进行两轮验证,将筛选出的miRNA与心肌肥厚相关指标进行相关性分析,并在高血压组患者中进行检测。结果高通量测序结果显示,与健康对照组比较,高血压合并LVH组患者有45个miRNA差异表达(P<0.05)。两轮验证后,确定6个miRNA差异表达(P<0.05),并且其表达水平与室间隔厚度、左心室后壁厚度均呈显著相关。其中miR-532-5p表达水平在高血压组患者中显著升高(P<0.05),在高血压合并LVH组患者中进一步升高(P<0.05);miR-939-5p和miR-155-5p表达水平在高血压组患者中显著降低(P<0.05),且在高血压合并LVH组患者中进一步降低(P<0.05)。结论老年男性高血压性LVH患者miR-532-5p和miR-1294表达水平显著升高,miR-939-5p、miR-222-3p、miR-25-5p和miR-155-5p表达水平显著降低。miR-532-5p、miR-939-5p和miR-155-5p可能与高血压的发生及进展为高血压性LVH有关。Objective To analyze the levels of plasma microRNA(miRNAs)in elderly male patients with hypertensive left ventricular hypertrophy(LVH),and screen the miRNAs related to the pathogenesis of hypertension and hypertensive LVH.Methods The elderly males who were referred to Chinese PLA General Hospital from January 2021 to November 2022 were divided into control group(43 cases),hypertension with LVH group(43 cases),hypertension without LVH group(40 cases).The plasma was collected.First,3 patients in hypertension with LVH group and 3 matched in control group were selected for highthroughput miRNA sequencing,and two rounds of validation were performed in sequencing samples and large samples using quantitative real time PCR(qRTPCR).The correlation between the screened miRNAs and related LVH indexes were analyzed;meanwhile,the plasma levels of the miRNAs were determined in the hypertension group.Results 45 miRNAs were found to be differentially expressed in patients with hypertensive LVH(P<0.05)and 6 miRNAs were further validated in the second round of test.In addition,the level of miR-532-5p was increased in the hypertension group(P<0.05),and much more pronounced in patients with hypertensive LVH(P<0.05).The levels of miR-939-5p and miR-155-5p were decreased in the hypertension group(P<0.05),and much more significant in patients with hypertensive LVH(P<0.05).Conclusions miR-532-5p and miR-1294 were significantly increased in elderly male patients with hypertensive LVH,and miR-939-5p,miR-222-3p,miR-25-5p and miR-155-5p were significantly decreased.miR-532-5p,miR-939-5p and miR-155-5p may be related to the pathogenesis of hypertension and hypertensive LVH.

关 键 词：高血压 左心室肥厚 老年 男性 微RNA 

分 类 号：R544.1[医药卫生—心血管疾病]