提高饲用抗菌肽稳定性的分子遗传设计策略  

Molecular genetic design strategies for improving stability of feed antimicrobial peptides

作　　者：王涛 庞有志[1,2] 祁艳霞 谭鹏[3] 周宸龙 张小辉 马曦 白跃宇[4] WANG Tao;PANG You-zhi;QI Yan-xia;TAN Peng;ZHOU Chen-long;ZHANG Xiao-hui;MA Xi;BAI Yue-yu

机构地区：[1]河南科技大学动物科技学院,河南洛阳471003 [2]洛阳市动物遗传育种重点实验室,河南洛阳471003 [3]中国农业大学动物科技学院,北京100193 [4]河南省动物卫生监督所,河南郑州450008

出　　处：《饲料研究》2023年第12期148-153,共6页Feed Research

基　　金：河南省自然科学基金项目(项目编号:202300410153);河南省科技攻关项目(项目编号:202102110088);河南省重大公益专项(项目编号:201300111200-05)。

摘　　要：饲用抗菌肽具有特殊的物理破膜杀菌机制而不易产生耐药性,成为抗生素替代品的研发热点。但天然存在或是自主设计的抗菌肽均面临在体内环境稳定性低的问题,因此采用多种策略优化抗菌肽的相关特性对缓解上述问题具有重要意义。文章概述近年来通过分子遗传学手段提高抗菌肽盐离子和蛋白酶稳定性的各种策略,分析分子遗传手段在提高抗菌肽稳定性方面的优势和局限性,为今后抗菌肽的设计提供思路。Feeding antimicrobial peptides have a special physical membrane breaking bactericidal mechanism and are not easy to produce drug resistance.However,naturally occurring or self-designed antimicrobial peptides are faced with the problem of low environmental stability in vivo.Therefore,it is of great significance to optimize the related properties of antimicrobial peptides by adopting various strategies to alleviate the above problems.The paper reviews various strategies to improve the stability of antimicrobial peptides salts and proteases by molecular genetic methods in recent years,analyzes the advantages and limitations of molecular genetic methods in improving the stability of antimicrobial peptides,to provide ideas for the design of antimicrobial peptides in the future.

关 键 词：抗菌肽 稳定性 耐盐 抗酶解 分子遗传设计 

分 类 号：S816.7[农业科学—饲料科学]