尘螨过敏原硝基化的位点选择性分析  

Analysis of site-selective nitration in house dust mite allergens

作　　者：田婧宜 杨方星[1,2] TIAN Jingyi;YANG Fangxing(Key Laboratory of Environment Remediation and Ecological Health,Ministry of Education,College of Environmental and Resource Sciences,Zhejiang University,Hangzhou,310058,China;Research Center for Air Pollution and Health,Zhejiang University,Hangzhou,310058,China)

机构地区：[1]浙江大学环境与资源学院环境健康研究所,污染环境修复与生态健康教育部重点实验室,杭州310058 [2]浙江大学空气污染与健康研究中心,杭州310058

出　　处：《环境化学》2023年第7期2273-2281,共9页Environmental Chemistry

基　　金：浙江省自然科学基金公益项目(LGF21B070007);浙江大学空气污染与健康研究中心项目(I21405-11)资助.

摘　　要：过敏原的硝基化会引起其致敏潜能的增强,进而带来更大的致敏性健康风险.过敏原蛋白质通常含有多个酪氨酸硝基化位点,分析过敏原硝基化的位点选择性是探究硝基化对过敏原致敏性影响的重要基础.本文以尘螨过敏原为研究对象,建立了基于超高效液相色谱-串联质谱(UPLC-MS/MS)同时定量分析3种尘螨过敏原(Der f 1、Der p 1和Der p 2)的13个酪氨酸位点硝基化程度的方法,并应用于分析3种尘螨过敏原在过氧亚硝酸盐硝基化作用下的位点选择性.结果表明,3种尘螨过敏原均发生了位点特异性的硝基化,Y195、Y37和Y92分别为Der f 1、Der p 1和Der p 2中反应活性最高的硝基化位点.尘螨过敏原位点选择性的硝基化表明,在评价硝基化尘螨过敏原的致敏性变化时应当考虑其位点特异性的硝基化状况.Nitration of allergens can enhance their allergenic potential,consequently contributing to higher allergenic health risks.Allergen proteins usually contain more than one tyrosine which could be nitrated.Analysis of site-selective nitration is critical for exploring the effect of nitration on allergenicity of allergen.In this study,a method was developed for the quantitative analysis of the nitration degrees of total 13 tyrosine sites of three house dust mite(HDM)allergens(Der f 1,Der p 1 and Der p 2)by ultra performance liquid chromatography-tandem mass spectrometry(UPLCMS/MS).The method was then applied to analyze the site-specific nitration of tyrosine in the three HDM allergens under nitration of peroxynitrite(ONOO-).The results showed that HDM allergens were nitrated by ONOO-and the reactions were site-selectivity.Y195,Y37 and Y92 were the most reactive nitration sites of Der f 1,Der p 1 and Der p 2,respectively.The site-selective nitration of HDM allergens indicated that site-specific nitration should be considered and included when assessment of the changes of their allergenicity caused by nitration.

关 键 词：尘螨过敏原 酪氨酸 硝基化 位点选择性 液相色谱-串联质谱. 

分 类 号：R392[医药卫生—免疫学] X51[医药卫生—基础医学]