脂多糖刺激的鸡巨噬细胞源外体对文昌鸡树突状细胞的活化作用  

Exosomes derived from lipopolysaccharide-activated macrophage cell line HD 11 cell induce the activation of dendritic cells from Wenchang chicken in vitro

作　　者：邢薿文 谢言 覃尧 XING Niwen;XIE Yan;QIN Yao(School of Animal Science and Technology,Hainan University,Haikou,Hainan 570228,China)

机构地区：[1]海南大学动物科技学院,海口570228

出　　处：《热带生物学报》2023年第4期433-440,共8页Journal of Tropical Biology

基　　金：国家自然科学基金(31802173);海南大学E类引进人才科研启动项目[KYQD(ZR)1937]。

摘　　要：为了探究脂多糖(Lipopolysaccharide,LPS)激活的鸡巨噬细胞来源的外体能否作为免疫佐剂应用于文昌鸡(Gallus domesticus)的疫病防控,通过超速离心法提取外体,应用透射电镜、粒径分析方法对外体进行鉴定;然后通过PKH67染料标记外体,将外体与文昌鸡骨髓来源的树突状细胞(dendritic cell,dc)共孵育,最后利用荧光显微镜观察树突状细胞对外体的摄取情况以及活化情况。结果表明,脂多糖刺激鸡巨噬细胞来源的外体可以被文昌鸡来源的树突状细胞摄取,并能促进树突状细胞的活化。It has been demonstrated that exosomes derived from macrophages can be used as adjuvants to enhance cellular immune response.An attempt was made to explore whether exosomes derived from lipopolysaccharide-activated chicken macrophages could be used as immune adjuvants for the potential prevention and control of epidemic diseases in Wenchang chicken.The exosomes were extracted by ultracentrifugation and identified by transmission electron microscopy and particle size analysis.Subsequently,the exosomes were labeled with PKH67 dye and co-incubated with bone marrow-derived dendritic cells from Wenchang chicken.The uptake and activation of the exosomes by the dendritic cells were observed under a fluorescence microscope.The results showed that the exosomes derived from chicken macrophage HD 11 cells stimulated with lipopolysaccharides were ingested by amphioxus-derived dendritic cells and promoted the activation of dendritic cells.

关 键 词：外体 巨噬细胞 树突状细胞 文昌鸡 脂多糖 

分 类 号：S851.4[农业科学—预防兽医学]