多肉植物东云系2个品种组培快繁技术初探  

Preliminary Study on Tissue Culture and Rapid Propagation of Two Succulents Varieties from Echeveria agavoides Lines

作　　者：陆琳[1,2,3] 张天东 柯燚 李涵 苗振[3] 拔利超 杨平 李仕柳 曹桦 LU Lin;ZHANG Tiandong;KE Yi;LI Han;MIAO Zhen;BA Lichao;YANG Ping;LI Shiiu;CAO Hua(Flower Research Institute,Yunnan Academy of Agricultural Sciences,Kunming 650100,China;National Engineering Research Center for Ornamental Horticulture,Kunming 650100,China;Yuxi Chenghua Biotechnology Co.,Ltd.,Yuxi 652500,China;Minhui(Fujian)Horticulture,Co.,Ltd.,Ningde 352000,China;Yuxi City Jiangchuan District Rural Industrial Development Center,Yuxi 652600,China)

机构地区：[1]云南省农业科学院花卉研究所,云南昆明650100 [2]国家观赏园艺工程技术研究中心,云南昆明650100 [3]玉溪澄花生物科技有限公司,云南玉溪652500 [4]闵卉(福建)园艺有限公司,福建宁德352000 [5]玉溪市江川区乡村产业发展中心,云南玉溪652600

出　　处：《山西农业科学》2023年第8期861-866,共6页Journal of Shanxi Agricultural Sciences

基　　金：福建省林业科技项目(2022FKJ27)。

摘　　要：以东云系多肉植物品种HBG和阿姆斯特壮为试验材料,对外植体选择、诱导培养、增殖培养、生根培养和炼苗移栽等关键技术进行对比分析,旨在总结出东云系多肉的组培快繁体系,为解决东云系多肉植物繁殖率低的问题提供科学依据。结果表明,以幼嫩花芽为外植体诱导效果最佳,非花期可选用嫩芽;适宜诱导东云系多肉植物愈伤组织及丛生芽的培养基为MS+0.6~0.8 mg/L 6-BA+0.3~0.4 mg/L NAA+30 g/L蔗糖+7 g/L琼脂,pH值5.8~6.0;适宜东云系多肉植物的增殖培养基为MS+0.4~0.6 mg/L 6-BA+0.2 mg/L NAA+30 g/L蔗糖+7 g/L琼脂,pH值5.8~6.0;适宜东云系多肉植物的生根培养基为MS+0.2~0.3 mg/L NAA+30 g/L蔗糖+7 g/L琼脂,pH值5.8~6.0;直接剪去生根苗根部后再进行扦插,能大大提高其成活率。In this study,using succulent varieties HBG and Armstrong from Echeveria agavoides as the experimental materials,comparative analysis of key technologies such as explant selection,induction culture,proliferation culture,rooting culture,and seedling transplant was conducted to summarize the tissue culture and rapid propagation system of succulent plants from Echeveria agavoides lines,and to provide scientific basis for solving the problems of low reproduction rate and leaf cutting rate of succulent plants from Echeveria agavoides lines.The results showed that young flower buds were the best explants for induction,and tender buds could be used in non-flowering period.The optimal culture medium for callus induction and multiple buds of succulent plants from Echeveria agavoides lines was MS+0.6-0.8 mg/L of 6-BA+0.3-0.4 mg/L of NAA+30 g/L of sucrose+7 g/L of agar,pH 5.8-6.0.The most suitable proliferation medium was MS+0.4-0.6 mg/L of 6-BA+0.2 mg/L of NAA+30 g/L of sucrose+7 g/L of agar,pH 5.8-6.0.The optimal rooting medium was MS+0.2-0.3 mg/L of NAA+30 g/L of sucrose+7 g/L of agar,pH 5.8-6.0.The survival rate could be greatly improved by cutting the root of rooting seedlings directly before planting.

关 键 词：东云系多肉 外植体 组织培养 快繁技术 

分 类 号：S682.33[农业科学—观赏园艺]