高温高湿环境暴露对重度放创复合伤小鼠损伤恢复的影响  

Effects of hot and humid environments exposure on recovery of mice subjected to severe radiation injury combined with skin-wound trauma

作　　者：汪国建 谭雨龙[2] 龙爽[1] 吕晓凡 赵娜[1] 冉新泽[1] 王军平[1] 王涛[1] Wang Guojian;Tan Yulong;Long Shuang;Lv Xiaofan;Zhao Na;Ran Xinze;Wang Junping;Wang Tao(Institute of Combined Injury of PLA,State Key Laboratory of Trauma,Burn and Combined Injury,Army Medical University(Third Military Medical University),Chongqing 400038,China;Department of Tropical Medicine,College of Preventive Medicine,Army Medical University(Third Military Medical University),Chongqing 400038,China)

机构地区：[1]陆军军医大学(第三军医大学)军事预防医学系全军复合伤研究所创伤、烧伤与复合伤国家重点实验室,重庆400038 [2]陆军军医大学(第三军医大学)军事预防医学系全军复合伤研究所热带医学教研室,重庆400038

出　　处：《中华损伤与修复杂志（电子版）》2023年第4期285-292,共8页Chinese Journal of Injury Repair and Wound Healing(Electronic Edition)

基　　金：国家自然科学基金面上项目(82172219);军队后勤科研项目(ALJ19J002、ZLJ22J020)。

摘　　要：目的研究重度放创复合伤(R-W-CI)小鼠暴露于高温高湿环境(HHE)不同时间对小鼠存活率、造血损伤恢复、创面愈合及其病理学的影响。方法HHE模拟实验舱参数设定为温度(36±0.5)℃,相对湿度(70±5)%,通过肛温测评小鼠HHE暴露的效果。采用60 Coγ射线6.0 Gy全身照射,建立小鼠重度骨髓型急性放射病模型;照射后1.0 h内用刀片制成体表面积2.0%皮肤创伤模型。两种模型小鼠均于致伤前1.0 h放入模拟舱,转运与照射均由便携式高温高湿模拟舱维持HHE,致伤完毕后置于高温高湿模拟舱1.0 h和7.0 h后取出按常规条件饲养。急性放射损伤小鼠分为4组,即正常对照组(NC组)、放射损伤组(R组)、放射损伤HHE暴露1.0 h组(R+HHE 1.0 h组)、放射损伤HHE暴露7.0 h组(R+HHE 7.0 h组)。观察不同HHE暴露时间(1.0 h和7.0 h)对损伤后小鼠肛温、存活率、体重及外周血变化的影响。重度放创复合伤小鼠于伤后给予常规补液、创面包扎或左氧氟沙星(LEV)抗菌治疗,分为7组,即正常对照组(NC组)、放创复合伤组(R-W-CI组)、放创复合伤左氧氟沙星治疗组(R-W-CI+LEV组)、放创复合伤HHE暴露1.0 h组(R-W-CI+HHE 1.0 h组)、放创复合伤HHE暴露7.0 h组(R-W-CI+HHE 7.0 h组)、放创复合伤HHE暴露1.0 h左氧氟沙星治疗组(R-W-CI+HHE 1.0 h+LEV组)、放创复合伤HHE暴露7.0 h左氧氟沙星治疗组(R-W-CI+HHE 7.0 h+LEV组)。观察不同HHE暴露时间小鼠存活率、体重、外周血、创面愈合以及病理学指标变化。结果模拟实验舱设定HHE条件对小鼠的体温能够产生显著影响,达到HHE效果。HHE不同暴露时间对重度骨髓型急性放射病小鼠的体重恢复有不同影响,但对存活率无影响。在无左氧氟沙星抗菌治疗时,R-W-CI+HHE 1.0 h组小鼠存活率(40%)显著优于R-W-CI组(20%)(χ^(2)=5.496,P=0.019),但与R-W-CI+HHE 7.0组(30%)无显著差异。左氧氟沙星抗菌治疗能显著提高R-W-CI+LEV组与R-W-CI+HHE 1.0+LEV组小鼠存活�Objective To investigate the effects of hot and humid environments(HHE)exposure with different time on the recovery of mice suffered from radiation injury combined with skin-wound trauma(R-W-CI)by evaluating survival rate,hematopoietic injury recovery and cutaneous wound healing.Methods The parameters of the HHE simulation experiment module were set as 36℃±0.5℃in temperature and 70%±5%in relative humidity,which were confirmed by detecting rectal temperature after mice were put in the module.The animal model of severe hematopoietic acute radiation sickness was established by mice with 6.0 Gy 60 Coγ-rays whole body irradiation.The animal model of severe R-W-CI was established with mice exposed to 60 Coγ-rays(6.0 Gy)followed by a 2.0%total-body-surface-area wounds within 1 h after irradiation.Mice from both models were set into HHE for 1 h before irradiation and transferred to one portable HHE simulation chamber during transportation and irradiation.The injured mice were continued to be put into HHE module for another 1.0 h or 7.0 h followed by maintaining conventional ambient temperature and humidity conditions.For hematopoietic acute radiation sickness model,the mice were divided into four groups:normal control(NC group),radiation injury(R group),radiation injury exposed to HHE for 1.0 h(R+HHE1.0 h group),radiation injury exposed to HHE for 7.0 h(R+HHE7.0 h group).Indexes including rectal temperature,survival rate,body weight and complete blood count were measured to estimate the effects of different HHE exposure time on the recovery of radiation injury.For R-W-CI model,routine fluid supplement and wound bandaging or levofloxacin(LEV)were implemented as routine supportive treatments.The mice were divided into seven groups:normal control(NC group),combined radiation-trauma injury(R-W-CI group),combined radiation-trauma injury treated with levofloxacin(R-W-CI+LEV group),combined radiation-trauma injury exposed to HHE for 1.0 h(R-W-CI+HHE 1.0 h group),combined radiation-trauma injury exposed to HHE for 7.0 h(

关 键 词：高温高湿环境 放创复合伤 造血 创面愈合 

分 类 号：R644[医药卫生—外科学]