黄条鰤npy基因克隆及其对饥饿再投喂的应答特性  

Molecular Cloning and Characterization of npy Gene and Its Response to Starvation-Refeeding Strategy in Seriola aureovittata

作　　者：邱夏君 王滨[2] 徐永江[2] 崔爱君 姜燕[2] QIU Xiajun;WANG Bin;XU Yongjiang;CUI Aijun;JIANG Yan(College of Fisheries and Life Sciences,Shanghai Ocean University,Shanghai 201306,China;Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences/Joint Laboratory for Deep Blue Fishery Engineering of Pilot National Laboratory for Marine Science and Technology(Qingdao),Qingdao 266071,China)

机构地区：[1]上海海洋大学水产与生命学院,上海201306 [2]中国水产科学研究院黄海水产研究所、青岛海洋科学与技术试点国家实验室深蓝渔业工程联合实验室,山东青岛266071

出　　处：《渔业科学进展》2023年第4期74-83,共10页Progress in Fishery Sciences

基　　金：青岛海洋科学与技术试点国家实验室“十四五”重大项目(2022QNLM30001-1);国家重点研发计划项目(2022YFD2401102);中国水产科学研究院基本科研业务费(2020TD47);农业农村部财政专项–海洋渔业生物资源收集与保藏项目;财政部和农业农村部:国家现代农业产业技术体系(CARS-47)共同资助。

摘　　要：为探究神经肽Y(neuropeptide Y,NPY)在黄条鰤(Seriola aureovittata)摄食调控中的作用及机制,本研究采用同源克隆的方法获得了黄条鰤npy基因的开放阅读框(ORF)序列,并利用实时荧光定量PCR技术分析了npy基因的组织分布以及其对饥饿再投喂的应答特性。黄条鰤npy基因ORF序列长度为300bp,编码99个氨基酸的前体蛋白,其中包括28个氨基酸的信号肽、36个氨基酸的成熟肽。氨基酸序列同源性比对发现,黄条鰤npy编码的氨基酸序列与斑马鱼(Daniorerio)等其他硬骨鱼高度保守(>90%);系统进化树分析表明,黄条鰤npy与高体鰤(Serioladumerili)的关系最近。npymRNA在所检测的12种组织中均有表达,其中,在脑组织表达量最高,在垂体和胃中表达量次之。在饥饿再投喂实验中,饥饿刺激了npymRNA的表达,特别是饥饿21d时,实验组垂体npy mRNA表达量显著高于对照组,再投喂7 d后恢复到对照组水平。上述结果表明,npy可能参与了黄条鰤的摄食调控,在饥饿代谢补偿机制中发挥了重要作用。Yellowtail kingfish(Seriola aureovittata),a pelagic marine finfish species with a worldwide distribution,is regarded as an emerging candidate for the aquaculture industry owing to its fast growth,superior flesh quality,and farming suitability in both sea cages and land-based facilities in China.The species has high economic value and is the second most produced Seriola species in the world following Japanese yellowtail Seriola quinqueradiata.Researchers worldwide have studied the role of regulatory factors neuropeptide Y(NPY)in fish feeding regulation.In recent years,there has been great progress in research on food intake in fish,however,very little attention has been paid to the endocrine regulation mechanism of food intake.Methods on strengthening the production performance of fish through appetite regulation is still a hot research topic.The control of food intake and energy metabolism in vertebrates are complex processes involving several neural pathways.Some hypothalamic signals are released by peripheral tissues that are associated with energy homeostasis or nutrient availability.Among the signaling molecules involved,NPY plays a key role.NPY is recognized as one of the most effective appetite regulators,which primarily function as a signaling factor to regulate a variety of biological processes such as food intake and glucose homeostasis.The orexigenic actions of NPY have been well investigated thoroughly over the past decades.Much evidence supports that NPY's functional role as a regulator of energy homeostasis and appetite control is conserved across vertebrates,including teleosts.In several species,including rainbow trout,Nile tilapia,and grass carp,NPY injections increase food intake,supporting an orexigenic role.In line with this,food deprivation increased npy mRNA expression in the brain,such as seen for goldfish and Johnny carp.Moreover,refeeding normalized npy mRNA abundance following food deprivation.As S.aureovittata feeds heavily and fiercely,the breeding industries need to understand its feedi

关 键 词：黄条鰤 NPY 克隆 组织表达 摄食 

分 类 号：S917.4[农业科学—水产科学]