黄条鰤igfbp-1和igfbp-2基因克隆、表达及其生长调控作用  

Molecular Cloning,Expression Profiles of Insulin-Like Growth Factor-Binding Protein-1(igfbp-1)and igfbp-2 and their regulation effects on growth of Yellowtail Kingfish(Seriola aureovittata)

作　　者：张文静 徐永江[2] 崔爱君 王滨[2] 姜燕[2] 王开杰 周鹤庭 ZHANG Wenjing;XU Yongjiang;CUI Aijun;WANG Bin;JIANG Yan;WANG Kaijie;ZHOU Heting(National Engineering Research Center for Marine Aquaculture,Zhejiang Ocean University,Zhoushan 316022,China;Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Joint Laboratory for Deep Blue Fishery Engineering,Pilot National Laboratory for Marine Science and Technology(Qingdao),Qingdao 266071,China)

机构地区：[1]浙江海洋大学国家海洋设施养殖工程技术研究中心,浙江舟山316022 [2]中国水产科学研究院黄海水产研究所、青岛海洋科学与技术试点国家实验室深蓝渔业工程联合实验室,山东青岛266071

出　　处：《渔业科学进展》2023年第4期84-98,共15页Progress in Fishery Sciences

基　　金：国家重点研发计划项目(2022YFD2401102);青岛海洋科学与技术试点国家实验室“十四五”重大项目(2022QNLM30001-1);中国水产科学研究院基本科研业务费(2020TD47);农业农村部财政专项–海洋渔业生物资源收集与保藏项目;财政部和农业农村部:国家现代农业产业技术体系(CARS-47)共同资助。

摘　　要：胰岛素样生长因子结合蛋白(insulin-like growth factor binding proteins,IGFBPs)在调节胰岛素样生长因子的生物学活性和生长调控中起着至关重要的生理作用。本研究克隆了黄条鰤(Seriola aureovittata)igfbp-1、igfbp-2a和igfbp-2b等3个基因cDNA编码的开放阅读框(ORF)序列,分析了其组织分布特征,并检测了工厂化不同养殖密度下黄条鰤肝脏中5个基因igfbp-1、igfbp-2a、igfbp-2b、igf-1和igf-2对生长的调控作用。结果显示,igfbp-1的ORF长为741 bp,共编码246个氨基酸;igfbp-2a的ORF长度为882 bp,共编码293个氨基酸;igfbp-2b的ORF长度为810 bp,共编码269个氨基酸。它们均具有广泛的组织表达特性,其中在肝脏中显著高表达,且具有性别二态性表达特性。工厂化养殖条件下,低密度组实验鱼生长速度最快且igfbp-1、igfbp-2a、igfbp-2b、igf-1和igf-2表达量均最高,与中、高密度组有显著性差异(P<0.05),且与血清IGF-1、GH表达趋势相同,与皮质醇含量及葡萄糖浓度的趋势相反;而中、高密度组实验鱼的生长及igfbp-1、igfbp-2a、igfbp-2b、igf-1和igf-2表达量均无显著差异。本研究表明,igfbp-1、igfbp-2a和igfbp-2b参与了不同养殖密度下黄条鰤生长的调控过程,且与igf-1、igf-2对生长的表达调控存在正向协同效应。研究结果为阐释黄条鰤生长的分子机制以及工厂化条件下适宜养殖密度的调控提供了理论依据。Insulin-like growth factor binding proteins(IGFBPs)play crucial roles in regulating biological activities of insulin-like growth factors(IGFs)and growth performance in vertebrates.IGFBP is a six-member protein family(IGFBP1–6)with a high affinity for IGF.It affects the distribution,stability,and biological activity of IGF by regulating the interaction between IGF ligands and receptors.Recently,IGFBP-1 was identified as a regulator of growth,reproduction,and development in bony fish,such as juvenile Atlantic salmon,where IGFBP-1 interacts with cortisol to regulate growth.IGFBP-1 regulates cell metabolism and growth through interaction with insulin in primary hepatocytes incubated in vitro in orange-spotted grouper(Epinephelus coioides).IGFBP-2 has a wide range of tissue expression characteristics in bony fish,and IGF regulation may occur through autocrine or paracrine pathways.For example,long-term fasting induced increased igfbp-2 mRNA expression in the liver of zebrafish.In addition,IGFBP-2 can inhibit the activity of IGF ligand through its high affinity with IGF to play an inhibitory and regulatory role in zebrafish growth.igfbp-2 mRNA expression was significantly up-regulated in Carassius auratus(goldfish)liver after fasting,and quickly recovered to normal levels after feeding.This indicated that igfbp-2 mRNA expression may be related to the anabolism and catabolism of goldfish,and is regulated by metabolic factors.Yellowtail kingfish(Seriola aureovittata)of the Carangidae family has high economic value and nutrition value.It is a warm and temperate oceanic fish with long distance migration characteristics that is distributed in the middle and upper oceans globally.This species is large with a fast growth rate,and is a promising candidate for aquaculture in land-based industrial circulating water system,deep-water cages,net pen systems,and aquaculture craft,etc.Our laboratory studied the regulatory mechanism of the rapid growth of yellowtail kingfish and cloned gh,igf-1,igf-2,ghr,and other growth-related fun

关 键 词：CDNA克隆 IGFBP-1 IGFBP-2 黄条鰤 组织表达 生长调控 

分 类 号：S965.3[农业科学—水产养殖]