GR24及氮磷供应对丹参-AM真菌共生体养分吸收及功能物质形成的影响  

Effects of GR24 and nitrogen and phosphorus supply on nutrient uptake and formation of functional substances in Salvia Miltiorrhiza-AMF symbiont

作　　者：孙颖盈 王欣雨 祝晨琳 朱梦圆 周端顼 金海如[1] SUN Ying-ying;WANG Xin-yu;ZHU Chen-lin;ZHU Meng-yuan;ZHOU Duan-xu;JIN Hai-ru(College of Chemistry and Life Science,Zhejiang Normal University,Jinhua,Zhejiang 321004,China;College of Forestry and Biotechnology,Zhejiang Agriculture and Forestry University,Hangzhou,Zhejiang 311302,China)

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321004 [2]浙江农林大学林业与生物技术学院,浙江杭州311302

出　　处：《植物营养与肥料学报》2023年第6期1150-1159,共10页Journal of Plant Nutrition and Fertilizers

基　　金：国家自然科学基金项目(41371291);浙江省公益技术应用研究计划项目(LGN20D010002)。

摘　　要：【目的】探究了外源GR24(人工合成独脚金内脂结构类似物)、磷水平和氮源对丹参–丛枝菌根(AM)真菌共生体氮素吸收及丹参酮类物质积累的影响,为丹参高质量生产提供参考。【方法】同位素示踪试验采用三室系统进行,试验设置3个磷水平[0μmol/L(P0)、35μmol/L(P35)、700μmol/L(P700)]和3个^(15)N标记氮源(硝酸钾、硫酸铵、尿素,^(15)N标记浓度均为4 mmol/L),分析丹参菌根中^(15)N丰度。GR24试验采用砂培方法进行,设置3种总氮浓度为4 mmol/L的氮源:硝酸钾、硫酸铵、尿素;设置3个AM真菌处理:不接种AM真菌(NM)、接种AM真菌(AM)、接种AM真菌+0.1 mmol/L GR24(AM+GR24),分析植株氮、磷积累量、氮磷比及丹参酮类物质的积累。【结果】同位素示踪试验结果显示,磷供应水平和氮源共同影响着丹参菌根对氮的吸收运转。硝酸钾处理下,磷供应水平对丹参菌根的^(15)N丰度无显著影响;硫酸铵处理下,提高磷的供应水平显著降低丹参菌根的^(15)N丰度,且P700相对于P0的降幅远远大于P35;尿素处理下,P35提高但P700急剧降低了丹参菌根中^(15)N丰度,P700的降幅远远大于P35的增幅。P0和P35水平下,尿素处理的丹参菌根^(15)N丰度显著高于硫酸铵和硝酸钾处理。外源GR24提高根系AM侵染率的效果仅限于丹参生长50天内。3个氮源处理下,AM接种均显著提高了丹参地上部和地下部生物量,且AM+GR24处理的丹参地上部生物量与AM处理无显著差异,而供应硝酸钾和尿素时,AM+GR24处理较AM处理进一步显著提高了根部的生物量。相比于NM,AM提高地上部和根部氮、磷积累量的效果不稳定,而AM+GR24处理显著提高了丹参地上部和根部氮、磷积累量。AM+GR24处理最有利于丹参地上部氮、磷积累的氮源分别是硫酸铵、尿素;最有利于根部氮、磷积累的氮源均是硝酸钾,其次是尿素。收获的菌根化丹参中,以供应硫酸铵的丹参酮IIA和隐丹参酮含�【Objectives】Exploring the effects of exogenous application of GR24 and N and P supply on the N uptake and tanshinones accumulation in the roots of Salvia miltiorrhiza-AMF symbionts.【Methods】The isotope experiment was conducted using a three chamber system,with three P levels:0μmol/L(P0),35μmol/L(P35),700μmol/L(P700),and three ^(15)N labeled N sources(KNO_(3),(NH_(4))_(2)SO_(4),urea,all with a ^(15)N labeled concentration of 4 mmol/L).The ^(15)N abundance in Salvia miltiorrhiza mycorrhiza were analyzed.The GR24 experiment was conducted using sand culture method,KNO_(3),(NH_(4))_(2)SO_(4),and urea were used as N sources and applied in the same N rate(4mmol/L)and three AMF treatments were set up under each N source,as:no inoculation of AMF(NM),inoculation of AMF(AM),and inoculation of AMF+0.1 mmol/L GR24(AM+GR24).The N and P contents,N/P ratio,and tanshinone substances in plants were measured.【Results】The results of isotope experiments showed that P supply level and N source jointly affected the absorption and transportation of N by Salvia miltiorrhiza mycorrhiza.Under KNO_(3) treatment,the P supply level did no significantly affect the abundance of ^(15)N in Salvia miltiorrhiza mycorrhiza;under(NH_(4))_(2)SO_(4) treatment,P supply significantly reduced the ^(15)N abundance of Salvia miltiorrhiza mycorrhiza,and the decrease in P700 was much greater than that in P35;under urea treatment,P35 increased but P700 sharply decreased the abundance of ^(15)N in Salvia miltiorrhiza mycorrhiza,the decrease in P700 was much greater than the increase in P35.At the P0 and P35 levels,the ^(15)N abundance of Salvia miltiorrhiza mycorrhizal fungi treated with urea was significantly higher than that with(NH_(4))_(2)SO_(4) and KNO_(3).The effect of exogenous GR24 on improving AMF colonization rate was limited within 50 days of Salvia miltiorrhiza growth.Under the three N source treatments,AM inoculation significantly increased the aboveground and underground biomass of Salvia miltiorrhiza.AM+GR24 did not further increas

关 键 词：GR24 氮 磷 丹参 AM真菌 同位素 丹参酮 

分 类 号：S567.53[农业科学—中草药栽培] S154.3[农业科学—作物学]