灯盏花素联合川芎嗪对NSCLC的抑制作用及其机制研究  

Inhibition of NSCLC by Combination of Breviscapine and Tetramethylpyrazine and its Mechanism

作　　者：史莉萍 刘地林 吕根梅 SHI Li-ping;LIU Di-lin;LYU Gen-mei(Department of Pharmocology,Gannan Health Vocational College,Ganzhou Jiangxi,341000,China;Department of Pharmocology,Ganzhou Cancer Hospital,Ganzhou Jiangxi,341000,China)

机构地区：[1]赣南卫生健康职业学院药学系,江西赣州341000 [2]赣州市肿瘤医院药剂科,江西赣州341000

出　　处：《中华养生保健》2023年第16期18-21,25,共5页CHINESE HEALTH CARE

基　　金：江西省教育厅科技计划项目(GJJ217104)。

摘　　要：目的探讨灯盏花素联合川芎嗪对NSCLC的抑制作用及其机制。方法将非小细胞肺癌A549细胞分为对照组,灯盏花素组(60μmol/L)、川芎嗪组(40μmol/L)、灯盏花素联合川芎嗪组(灯盏花素:60μmol/L;川芎嗪:40μmol/L),使用MTT法检测细胞存活率、流式细胞术检测细胞凋亡、Transwell试验检测细胞迁移能力、Western Blot实验检测相关通路变化。结果对照组、灯盏花素组、川芎嗪组、灯盏花素联合川芎嗪组A549细胞24 h后的细胞存活率依次呈降低趋势(P<0.05);与对照组相比,给药后的A549细胞出现明显的凋亡情况,且灯盏花素和川芎嗪联合用药组肺癌细胞凋亡最明显;Transwell实验结果显示,与对照组相比,给药后穿膜细胞数量显著减少,且灯盏花素联合川芎嗪抑制细胞穿膜效果最显著;Western Blot实验显示,给药后p-JAK2和p-STAT3的表达降低,且灯盏花素联合川芎嗪组中两种蛋白表达量最低。结论灯盏花素联合川芎嗪可抑制非小细胞肺癌细胞系A549的增殖、迁移和侵袭,其机制可能与JAK-STAT通路被抑制有关。Objective To investigate the mechanism of inhibition of NSCLC by combination of Breviscapine and Tetramethylpyrazine.Methods Non-small cell lung cancer A549 cells were divided into control group,Breviscapine group,Tetramethylpyrazine group,Breviscapine and Tetramethylpyrazine group.Detect cell survival rate by MTT,apoptosis by fl ow cytometry,cell migration ability by Transwell test.Related pathway changes were detected by Western Blot.Results The cell survival rate of A549 cells in the control group,Breviscapine group,Tetramethylpyrazine group,and Breviscapine and Tetramethylpyrazine group decreased after 24h(P<0.05).Compared with control group,A549 cells showed signifi cant apoptosis and cells in Breviscapine and Tetramethylpyrazine group showed the most evident changes;Transwell showed that the number of trans-membranous cells decreased signifi cantly compared with the control group and membrane penetration was most evidently inhibited in combination group.Western blot showed that the expression of p-JAK2 and p-STAT3 decreased after drug administration,and the two proteins were lowest in the Breviscapine and Tetramethylpyrazine group.Conclusion Breviscapine combined with Tetramethylpyrazine inhibited the proliferation,migration and invasion of the non-small cell lung cancer cell line A549,and the mechanism may be related to the inhibition of the JAK-STAT pathway.

关 键 词：灯盏花素 川芎嗪 非小细胞肺癌 

分 类 号：R285[医药卫生—中药学]