五种茄科植物COLD1基因生物信息学分析  

Bioinformatics Analysis of COLD1 Gene in Five Solanaceae Plants

作　　者：王文文[1] 王美 张丽[2] 郭新勇[1] Wang Wenwen;Wang Mei;Zhang Li;Guo Xinyong(College of Life Sciences,Shihezi University,Shihezi,832061;Shihezi University School of Medical,Shihezi,832061)

机构地区：[1]石河子大学生命科学学院,石河子832061 [2]石河子大学医学院,石河子832061

出　　处：《分子植物育种》2023年第15期4895-4901,共7页Molecular Plant Breeding

基　　金：石河子大学高层次人才科研启动项目(RCZK202045);第三师图木舒克市财政科技计划项目(KY2021GG13);地区科学基金项目(32160071)共同资助。

摘　　要：G蛋白作为植物中重要的信号传导因子,在对抗非生物胁迫方面具有重要作用。其相关蛋白COLD1能够调控植物耐寒性,在一些禾本科和十字花科抵御低温冷害过程中发挥了积极作用。本研究以加工番茄(Lycopersicon esculentum Mill.)为材料,利用PCR克隆出COLD1基因,通过BLAST序列比对获得了其他四种茄科植物中的同源基因。对其基因编码的蛋白质进行生物信息学分析发现,五种COLD1编码的蛋白质序列中亮氨酸含量最高,并预测这些蛋白均含有信号肽序列,具九次跨膜结构域,属于疏水蛋白,这些相同点意味着不同植物耐寒机理类似;但在氨基酸序列、理化性质和磷酸化位点等方面存在一定差异,差异性表明不同植物耐寒性不同。本研究为提高作物的耐寒性或培育耐寒性的作物提供一定的参考。As an important signal transduction factor in plants,G protein plays an important role in enhancing abiotic stress.COLD1 is a regulating cold tolerance gene and plays a positive role in the process of resisting cold injury in some Gramineae and Cruciferae plants.We used PCR to clone COLD1 in the processing tomato(Lycopersicon esculentum Mill.),than obtained its homologous genes in other four Solanaceae plants by BLAST.Bioinformatics analysis of the proteins encoded by COLD1 genes predicted that they contain the most leucine and signal peptide sequence,besides,there are nine times of the membrane structure domain and hydrophobic.These similarities mean that different plants have similar cold tolerance mechanism.However,there are also some differences,such as amino acid sequence,physical and chemical properties,phosphorylation sites.These indicate that different plants have different cold tolerance.This study provides some reference for improving the cold tolerance of crops and cultivating cold tolerance crops.

关 键 词：COLD1 茄科植物 耐寒性 生物信息学分析 

分 类 号：Q943.2[生物学—植物学]