大丽轮枝菌侵染棉花幼苗过程中内源水杨酸响应的初步分析  

Response of Endogenous Salicylic Acid in Cotton Seedlings Infected by Verticillium dahliae

作　　者：宁硕 顾爱星[1] 张国丽[3] 邓亚辉 陈全家[1] 孙国清[2] Ning Shuo;Gu Aixing;Zhang Guoli;Deng Yahui;Chen Quanjia;Sun Guoqing(Key Laboratory of Agricultural Technology,Cotton Engineering Research Center,College of Agriculture,Xinjiang Agricultural University,Urumqi,830052;Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing,100081;Biotechnology Research Institute,Xinjiang Academy of Agricultural and Reclamation Sciences,Shihezi,832000)

机构地区：[1]新疆农业大学农学院,棉花教育部工程研究中心,农业生物技术重点实验室,乌鲁木齐830052 [2]中国农业科学院生物技术研究所,北京100081 [3]新疆农垦科学院生物技术研究所,石河子832000

出　　处：《分子植物育种》2023年第15期5079-5087,共9页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31771851);新疆维吾尔自治区博士后科研流动站共同资助。

摘　　要：大丽轮枝菌(Verticillium dahliae)会引起棉花黄萎病,是棉花可持续发展的主要障碍之一。为了揭示棉花幼苗对真菌病原的免疫反应途径,本研究利用带有增强型绿色荧记s GFP的大丽轮枝菌Vd592-G对5个不同品种棉花进行侵染,并对侵染发生后不同时间不同部位的棉花组织进行内源水杨酸含量的测定。结果表明:(1)不同棉花品种、不同取样部位对植株内源性水杨酸含量存在极显著(P<0.01)的影响,但两者的交互性不显著。(2)相同部位的不同棉种间水杨酸含量不存在显著差异,相同棉种间不同部位的水杨酸含量不存在显著差异,两者的交互作用不显著;但是同一棉种间的根、茎、叶接种后内源水杨酸含量变化呈现极显著相关(P<0.01)。(3)属抗黄萎病类型的棉花植株水杨酸含量显著小于属耐黄萎病类型的棉花植株(P=0.033)。(4)不同抗病性品种的植株水杨酸含量的差异性主要体现在根部。(5)同一抗病类型的棉花植株中根、茎、叶的水杨酸含量变化呈现极显著相关,不同抗病类型的棉花植株中只有茎部位的水杨酸含量存在显著相关。(6)‘海7124’茎部和‘新海14号’茎部的水杨酸含量变化存在极显著相关、‘新海21号’茎部和‘新海14号’茎部的水杨酸含量呈现显著相关性。(7)‘海7124’和‘中植棉2号’的根部、‘海7124’和‘中植棉2号’的叶片、‘新海14号’和‘中植棉2号’的叶片以及‘新陆早36号’和‘中植棉2号’的叶片水杨酸含量变化存在显著差异。(8)在植株内源水杨酸含量变化过程中,抗病性与棉花品种、取样部位具有极显著的交互性,强于抗病性能与棉种间交互作用。本研究揭示了大丽轮枝菌侵染棉花幼苗时,不同棉种(海岛棉,陆地棉)、不同抗病性(抗病,感病)、不同部位(根,茎,叶)内源水杨酸的一些响应规律,为进一步挖掘抗病基因和构建抗病激素响应网络的研究�Verticillium dahliae is one of the main obstacles to the sustainable development of cotton.sustainable development of cotton.In order to reveal the immune response pathway of cotton seedlings to fungal pathogens,Verticillium dahliae Vd592-G with enhanced green fluorescent marker sGFP was used to infect five different varieties of cotton,and the content of Endogenous Salicylic Acid in different parts of cotton tissues at different times after infection was determined.The results showed that:(1)Different cotton varieties and sampling parts had a very significant effect on the content of Endogenous Salicylic Acid(P<0.01),but the interaction between them was not significant.(2)There was no significant difference in salicylic acid content between different cotton species in the same part,there was no significant difference in salicylic acid content between different parts of the same cotton species,and the interaction between them was not significant;However,there was a significant correlation between the changes of Endogenous Salicylic Acid Content in roots,stems and leaves of the same cotton species(P<0.01).(3)The salicylic acid content of cotton plants with Verticillium wilt resistance was significantly lower than that of cotton plants with Verticillium wilt resistance(P=0.033).(4)The difference of salicylic acid content in plants of different disease resistant varieties is mainly reflected in the roots.(5)The changes of salicylic acid content in roots,stems and leaves of cotton plants with the same disease resistance type showed a very significant correlation.In cotton plants with different disease resistance types,only the salicylic acid content in stems was significantly correlated.(6)There was a very significant correlation between the changes of salicylic acid content in the stem of Hai7124 and Xinhai14,and there was a significant correlation between the stem of Xinhai21 and Xinhai14.(7)There were significant differences in salicylic acid content among hai7124 root zhongzhimian2 root,hai7124 leaf zhongzhimian2

关 键 词：棉花 黄萎病 大丽轮枝菌 水杨酸 

分 类 号：S435.621.2[农业科学—农业昆虫与害虫防治]