粉葛生理特性对干旱胁迫的响应  

Responses of Physiological Characteristics of Pueraria montana var.Thomsonii to Drought Stress

作　　者：李鑫 敖志超 周洁 蔡金文 满建民[1] Li Xin;Ao Zhichao;Zhou Jie;Cai Jinwen;Man Jianmin(Key Laboratory of Mountain Plant Resources Protection and Germplasm Innovation Ministry of Education,College of Life Sciences,Guizhou University,Guiyang,550025;Jiangsu Key Life Science and Technology Development Co.,Ltd.,Jiangsu,224100)

机构地区：[1]贵州大学生命科学学院/山地植物资源保护与种质创新教育部重点实验室,贵阳550025 [2]江苏菌钥生命科技发展有限公司,江苏224100

出　　处：《分子植物育种》2023年第15期5110-5121,共12页Molecular Plant Breeding

基　　金：贵州省教育厅青年科技人才成长项目(黔教合KY字[2016]127);贵州省自然科学基金项目(黔科合基础[2016]1050);贵州省中药材现代产业技术体系建设项目(GZCYTX2021-02)共同资助。

摘　　要：为探究粉葛(Pueraria montana var. thomsonii)对干旱胁迫的生理响应机制,本研究以粉葛植株为试验材料,通过盆栽形式和自然耗水与人工补水相结合的控水方法,分别设定了3个不同水分梯度。结果表明:干旱胁迫会抑制粉葛生长,叶片相对含水量和根系活力降低,叶绿素总含量显著增加;超氧化物歧化酶(SOD)和过氧化物酶(POD)酶活性均得到提高,过氧化氢酶(CAT)酶活性在中度干旱胁迫下逐渐增加,重度干旱胁迫下CAT酶活性逐渐降低;叶肉细胞相对电导率显著增加,渗透调节物质浓度增加;总黄酮含量显著升高。单叶面积与POD、叶绿素a、叶绿素b、总叶绿素含量和可溶性蛋白含量呈现显著负相关,POD和脯氨酸与总黄酮含量显著正相关。通过相关性分析与主成分分析可知,POD、相对含水量、SOD、类胡萝卜素、可溶性蛋白、CAT和总黄酮在干旱胁迫下表现为较敏感的生理指标。本研究结果可为粉葛节水及优质栽培的生理机制提供理论基础。In order to explore the physiological response mechanism of Pueraria montana var.Thomsonii to drought stress,in this study,Pueraria montana var.Thomsonii plants were used as test materials,and three different water gradients were set through the potted form and the water control method combining natural water consumption and artificial water supplement.The results showed that drought stress inhibited the growth of Pueraria montana var.Thomsonii,decreased leaf relative water content and root activity,and increased total chlorophyll content.The activities of superoxide dismutase(SOD)and peroxidase(POD)were increased,the activities of catalase(CAT)were increased under moderate drought stress,decreased under severe drought stress.The relative conductivity of mesophyll cells increased significantly,and the concentration of osmotic adjustment substances increased.Total flavonoids content increased significantly.Single leaf area was significantly negatively correlated with POD,chlorophyll a,chlorophyll b,total chlorophyll content and soluble protein content,while POD and proline were significantly positively correlated with total flavonoids content.Correlation analysis and principal component analysis showed that POD,relative water content,SOD,carotenoids,soluble protein,CAT and total flavonoids were sensitive physiological indexes under drought stress.The results can provide theoretical basis for the physiological mechanism of water-saving and high-quality cultivation of Pueraria montana var.Thomsonii.

关 键 词：粉葛(Pueraria montana var.thomsonii) 干旱胁迫 抗逆生理 

分 类 号：S567.19[农业科学—中草药栽培]