红树植物老鼠簕内生真菌橘青霉P4-5-1中的1个新异喹啉酮生物碱  

A new isoquinolone alkaloid from Penicillium citrinum P4-5-1,an endophytic fungus isolated from mangrove plant Acanthus ilicifolius

作　　者：张岗 陈忠茂 阮加陈 许莉 李国强[3] 罗联忠 ZHANG Gang;CHEN Zhongmao;RUAN Jiachen;XU Li;LI Guoqiang;LUO Lianzhong(Xiamen Key Laboratory of Marine Natural Product Resources,Xiamen Medical College,Xiamen 361023,China;Marine Biomedical Resources,Fujian University Engineering Research Center,Xiamen Medical College,Xiamen 361023,China;Key Laboratory of Marine Drugs,Ministry of Education,School of Medicine and Pharmacy,Ocean University of China,Qingdao 266003,China)

机构地区：[1]厦门医学院厦门市海洋药用天然产物资源重点实验室,福建厦门361023 [2]厦门医学院海洋生物医药资源福建省高等学校应用技术工程中心,福建厦门361023 [3]中国海洋大学海洋药物教育部重点实验室,医药学院,山东青岛266003

出　　处：《中国海洋药物》2023年第3期31-35,共5页Chinese Journal of Marine Drugs

基　　金：福建省高等学校科技创新团队项目(闽教科[2018]49号);福建省大学生创新创业训练计划项目(201912631018)资助。

摘　　要：目的研究老鼠簕内生真菌橘青霉(Penicillium citrinum)P4-5-1的化学成分。方法利用正反相硅胶柱色谱、Sephadex LH-20凝胶柱色谱以及半制备高效液相色谱(HPLC)等技术对P4-5-1次生代谢产物进行分离,利用高分辨质谱、一维及二维核磁共振等波谱鉴定技术确定化合物结构。结果从P4-5-1大米发酵物中分离得到了6个化合物,分别为4-methoxy-2-methylisoquinolin-1(2H)-one(1)、fischexanthone(2)、penicitrinone A(3)、ent-aspergoterpenin C(4)、sydowiol E(5)和citrinin(6)。结论化合物1为新异喹啉酮生物碱,命名为citrinadin D;化合物2、4和5首次从该种真菌中获得。Objective To study the chemical constituents of Penicillium citrinum P4-5-1,an endophytic fungus isolated from Acanthus ilicifolius.Methods Silica gel column chromatography,ODS reversed phase column chromatography,Sephadex LH-20 chromatography and semi-preparative high performance liquid chromatography were used to seperate the secondary metabolites of P4-5-1,and the high resolution mass spectrometry(HR-MS)and nuclear magnetic resonance spectroscopy(1D-and 2D-NMR)were employed to identify structures of isolates.Results A total of 6 compounds were obtained from the EtOAc extract of the fungal rice culture and identified as 4-methoxy-2-methylisoquinolin-1(2H)-one(1),fischexanthone(2),penicitrinone A(3),ent-aspergoterpenin C(4),sydowiol E(5)and citrinin(6),respectively.Conclusion Compound 1 was a new isoquinolone alkaloid and named citrinadin D.Compounds 2,4 and 5 were obtained from this fungus for the first time.

关 键 词：老鼠簕 内生真菌 橘青霉 化学成分 结构鉴定 

分 类 号：R931.77[医药卫生—生药学]