基于类酶活性二氧化锰纳米片的新型传感器应用于血清胆固醇的高灵敏特异性检测  

A novel sensor based on manganese dioxide nanosheets with enzyme-like activity for highly sensitive and specific detection of cholesterol in serum

作　　者：赖荣基 汪贝贝 陈婷婷 覃英凤 刘金文 Lai Rongji;Wang Beibei;Chen Tingting;Qin Yingfeng;Liu Jinwen(Department of Biochemistry and Molecular Biology,School of Basic Medical Sciences,The Key Laboratory of Biological Molecular Medicine Research in Guangxi Universities,Guangxi Medical University,Nanning 530021,China;Key Laboratory of Longevity and Aging-related Diseases Ministry of Education,Guangxi Medical University,Nanning 530021,China;Department of Human Anatomy,School of Basic Medical Sciences,Guangxi Medical University,Nanning 530021,China)

机构地区：[1]广西医科大学基础医学院生物化学与分子生物学教研室、广西高校生物分子医学研究重点实验室,南宁530021 [2]广西医科大学长寿与老年相关疾病教育部重点实验室,南宁530021 [3]广西医科大学基础医学院人体解剖学教研室,南宁530021

出　　处：《广西医科大学学报》2023年第6期1055-1061,共7页Journal of Guangxi Medical University

基　　金：国家自然科学基金资助项目(No.22266007);广西高校中青年教师科研基础能力提升项目(No.2022KY0098)。

摘　　要：目的:研究基于类酶活性二氧化锰纳米片(MnO_(2)NS)的新型传感应用于血清中胆固醇(Chol)的检测效果。方法:以四甲基氢氧化铵、过氧化氢和氯化锰为原料制备得到MnO_(2)NS。利用Chol和胆固醇氧化酶(ChOx)的氧化反应和MnO_(2)NS催化3,3’,5,5’-四甲基联苯胺(TMB)显色反应,根据反应后溶液颜色及吸光度的变化对Chol进行定量检测,并对反应温度、pH以及反应时间等条件进行了优化。结果:成功合成了类酶活性的MnO_(2)NS。该方法用于Chol的检测比传统MnO_(2)NS比色法具有更优的分析性能。在最优的实验条件下,Chol的检测线性范围为1~200μmol/L,检测限为0.5μmol/L,且具有较高的选择性。此外,该方法用于人血清中Chol的检测结果令人满意。结论:该传感器操作简单、快捷,具有较高的特异性与灵敏度,可为Chol相关的临床诊断提供新的检测方法。Objective:To investigate the effect of a novel sensor based on manganese dioxide nanosheets(MnO_(2)NS)with enzyme-like activity for the detection of cholesterol(Chol)in human serum.Methods:MnO_(2)NS was prepared using tetramethylammonium hydroxide,hydrogen peroxide and manganese chloride as raw materials.The oxidation reaction of Chol and cholesterol oxidase(ChOx)and the colorimetric reaction of 3,3’,5,5’-tetramethylbenzidine(TMB)catalyzed by MnO_(2)NS were used to detect Chol quantitatively according to the changes in the color and the absorbance of the solution,and the reaction temperature,pH and reaction time were optimized.Results:MnO_(2)NS with enzyme-like activity was successfully synthesized.Compared to the traditional MnO_(2)NS colorimetric method,this method for Chol detection had better analytical performance.Under the best experimental conditions,the linear detection range was from 1μmol/L to 200μmol/L for Chol,with a detection limit of 0.5μmol/L and high selectivity.In addition,the method had achieved satisfactory results for Chol detection in human serum.Conclusion:The sensor is simple and easy to operate,with high specificity and sensitivity,providing a new detection method for Chol-related clinical diagnosis.

关 键 词：胆固醇 胆固醇氧化酶 比色分析法 二氧化锰纳米片 3 3’ 5 5’-四甲基联苯胺 

分 类 号：R446.11[医药卫生—诊断学]