预防性输卵管切除组织中突变型P53蛋白表达情况及意义的探讨  

Study for Expression and Significance of Mutant P53 Protein in the Tissues of Prophylactic Salpingectomy

作　　者：郭清烽 毋思思 陈加荣 闫国良[2] 张然[2] 刘红丽 GUO Qingfeng;WU Sisi;CHEN Jiarong;YAN Guoliang;ZHANG Ran;LIU Hongli(Xiamen Maternal and Child Health Care Hospital,Xiamen 361003,China;不详)

机构地区：[1]厦门市妇幼保健院(厦门大学附属妇女儿童医院),福建厦门361003 [2]厦门大学医学院

出　　处：《中外医学研究》2023年第21期75-81,共7页CHINESE AND FOREIGN MEDICAL RESEARCH

基　　金：2020年厦门市医疗卫生指导性项目(3502Z20209208);厦门大学大学生创新创业训练计划项目(2021X1064)。

摘　　要：目的:探讨预防性输卵管切除组织中突变型P53蛋白表达情况及意义。方法:收集2014年5月—2018年9月厦门市妇幼保健院因子宫良性病变行腹腔镜下全子宫或次全切除术,同时行双侧输卵管切除术的929例患者,其中腹腔镜下全子宫切除术+双侧输卵管切除术827例,腹腔镜下次全切除术+双侧输卵管切除术102例。应用免疫组化法检测突变型P53蛋白在输卵管组织中的表达情况,分析影响突变型P53蛋白表达的因素,并随访术后患者浆液性卵巢癌的发生情况。结果:不同输卵管组织中突变型P53蛋白表达情况患者子宫良性疾病类型、输卵管结扎史、剖宫产史和年龄比较,差异无统计学意义(P>0.05);但不同输卵管组织中突变型P53蛋白表达情况患者糖类抗原125(CA125)水平比较,差异有统计学意义(P<0.05)。logistic回归分析显示,CA125阳性是突变型P53蛋白阳性表达的危险因素(P<0.05)。全子宫+双侧输卵管切除及次全子宫+双侧输卵管切除术后患者突变型P53蛋白阳性组中未出现浆液性卵巢癌。结论:预防性输卵管切除组织中突变型P53蛋白表达阳性率为14.32%,CA125阳性是突变型P53蛋白阳性表达的危险因素,输卵管切除患者术后无浆液性卵巢癌的发生。Objective:To investigate the expression and significance of mutant P53 protein in the tissues of prophylactic salpingectomy.Method:A total of 929 patients who underwent laparoscopic total or subtotal hysterectomy and bilateral salpingectomy for benign uterine lesions in Xiamen Maternal and Child Health Care Hospital from May 2014 to September 2018 were collected,there were 827 cases of laparoscopic total hysterectomy+bilateral salpingectomy and 102 cases of laparoscopic subtotal hysterectomy+bilateral salpingectomy.The expression of mutant P53 protein in fallopian tube tissue was detected by immunohistochemistry.The factors affecting the expression of mutant P53 protein was analyzed,and followed up the occurrence of serous ovarian cancer after surgery.Result:There were no statistically significant differences in the expression of mutant P53 protein in different fallopian tube tissues among patients with benign uterine disease type,tubal ligation history,cesarean section history and age(P>0.05);however,there was significant difference in the expression of mutant P53 protein in different fallopian tube tissues among patients with the levels of carbohydrate antigen 125(CA125)(P<0.05).logistic regression analysis showed that CA125 positive was a risk factor for mutant P53 positive protein expression(P<0.05).There was no serous ovarian cancer in the mutant P53 protein positive group after total uterus+bilateral salpingectomy and subtotal uterus+bilateral salpingectomy.Conclusion:The positive rate of mutant P53 protein expression in the tissues of prophylactic salpingectomy was 14.32%,and CA125 positive was a risk factor for mutant P53 positive protein expression,no serous ovarian cancer occurred in these patients after postoperative follow-up.

关 键 词：子宫切除 输卵管切除 浆液性卵巢癌 免疫组织化学 

分 类 号：R713.51[医药卫生—妇产科学]