旱黄瓜茎基腐病菌鉴定及其生物学特性  

Identification and Biological Characterization of Pathogen Causing Stem Basal Rot of Cucumber

作　　者：金鸽 闵康 陈怡铭 曹蜢 张宇萍 韩亚梅 高玉峰 贺字典 JIN Ge;MIN Kang;CHEN Yiming;CAO Meng;ZHANG Yuping;HAN Yamei;GAO Yufeng;HE Zidian(College of Agronomy and Biotechnology,Hebei Key Laboratory of Crop Stress Biology,Hebei Normal University of Science&Technology,Qinhuangdao HeBei,066004,China;Journal Editorial Department,Hebei Normal University of Science&Technology,Qinhuangdao HeBei,066004,China)

机构地区：[1]河北科技师范学院农学与生物科技学院河北省作物逆境生物学重点实验室,河北秦皇岛066004 [2]河北科技师范学院学报编辑部,河北秦皇岛066004

出　　处：《河北科技师范学院学报》2023年第2期12-18,共7页Journal of Hebei Normal University of Science & Technology

基　　金：河北省重点研发项目(项目编号:21326516D,22326501D);河北省现代农业产业技术体系露地蔬菜创新团队项目(项目编号:HBCT2021200206);河北省研究生创新资助项目(项目编号:CXZZ202206)。

摘　　要：为明确昌黎旱黄瓜茎基腐病的病原菌,采用组织分离法和针刺法对其病原物进行分离培养和致病性测定,通过形态学、ITS序列分析方法鉴定旱黄瓜茎基腐病原菌种类后对病原菌的生物学特性进行了研究。结果表明:旱黄瓜茎基腐病的病原菌为拟轮枝镰孢菌(Fusarium verticillioides);该病菌菌丝最适生长温度为30℃,最适pH为6.0,最适碳源为葡萄糖,最适氮源为NH_(4)NO_(3),致死温度为69℃,10 min;大型分生孢子生长最适温度为25℃,小型分生孢子生长最适温度为30℃;两种孢子生长的最适pH,碳源,氮源均为5.0,蔗糖,NH_(4)NO_(3)。The pathogen was isolated and cultured with tissue isolation method and the pathogenicity of pathogen was determined with acupuncture method.The biological characterization of pathogen was studied after the pathogen variety causing stem basal rot of cucumber in Changli County was identified with morphology and IIS sequence analysis.The results showed that the pathogen of cucumber stem basal rot was Fusarium verticillioides.The optimum temperature,pH,carbon source,nitrogen source and lethal temperature for F.verticillioides mycelial growth were 30℃,6.0,glucose,ammonium nitrate,69℃and 10 min,respectively.The optimum growth temperature of macroconidia was different from microconidia,25℃and 30℃,respectively.The optimum pH,carbon source and nitrogen source of macroconidia and microconidia were both 5.0,sucrose,and ammonium nitrate.

关 键 词：旱黄瓜茎基腐病 致病性 生物学特性 拟轮枝镰孢菌(Fusarium verticillioides) 

分 类 号：S436.421.1[农业科学—农业昆虫与害虫防治]