麻风树茎枝中抑制小胶质细胞过度活化的活性成分研究  

Study on active compounds inhibiting microglia overactivation in stems of Jatropha curcas L.

作　　者：倪雪 王营杰 周地 侯悦 谢海辉[3] 吴国江[3] 李宁[1] NI Xue;WANG Yingjie;ZHOU Di;HOU Yue;XIE Haihui;WU Guojiang;LI Ning(School of Traditional Chinese Materia Medica,Shenyang Pharmaceutical University,Key Laboratory for TCM Material Basis Study and Innovative Drug Development of Shenyang City,Shenyang 110016,China;College of Life and Health Sciences,Northeastern University,Shenyang 110004,China;South China Botanical Garden,Chinese Academy of Sciences,Guangzhou 510650,China)

机构地区：[1]沈阳药科大学、中药学院、沈阳市中药药效物质研究与创新药开发重点实验室,辽宁沈阳110016 [2]东北大学生命科学与健康学院,辽宁沈阳110004 [3]中国科学院华南植物园,广东广州510650

出　　处：《沈阳药科大学学报》2023年第6期715-724,共10页Journal of Shenyang Pharmaceutical University

基　　金：国家自然科学基金资助项目(81872768,U1903122);沈阳市“中青年科技创新人才计划”资助项目(RC200408);辽宁省博士科研启动计划资助项目(2020-BS-129)。

摘　　要：目的对麻风树Jatropha curcas L.茎枝活性部位进行化学成分研究,并评价其对脂多糖(LPS)激活的BV-2小胶质细胞的抑制作用。方法采用硅胶、ODS、Sephadex LH-20柱色谱和半制备型HPLC等色谱方法进行分离纯化,运用多种波谱手段对化合物进行结构鉴定,并研究单体化合物对LPS激活的BV-2小胶质细胞释放一氧化氮(NO)的抑制作用。结果从麻风树茎枝正丁醇萃取物中鉴定出25个化合物,其中属中首次分离的化合物12个。化合物7和10表现出显著的抑制活性,其IC50值分别为(13.30±2.48)和(33.83±1.84)μmol·L^(-1),优于阳性药(米诺环素70.02±2.77μmol·L^(-1)),同时在活性浓度下未表现出细胞毒性。结论此研究进一步丰富了麻风树的化学成分,为深入研究麻风树药效物质及药理作用机制奠定了基础。Objective To study the chemical compounds from the leaves of Jatropha curcas L.and evaluate their inhibition on lipopolysaccharide(LPS)-activated BV-2 microglia.Methods The compounds were isolated and purified by silica gel,ODS,Sephadex LH-20 column chromatography and semi-preparative HPLC.The structures of the compounds were identified by various spectroscopic methods,and their inhibitory effects on nitric oxide(NO)release by LPS-activated BV-2 microglia were evaluated.Results 25 compounds were identified from the n-butanol extract of stems of J.curcas,of which 12 compounds were isolated from genus Jatropha for the first time.Besides,compounds 7 and 10 exhibited significant inhibitory activity with IC50 values of(13.30±2.48)and(33.83±1.84)μmol·L^(-1),respectively,which were superior to the positive drug[minocycline(70.02±2.77)μmol·L^(-1)]and showed no cytotoxicity at the active concentration.Conclusion This study further enriched the chemical compounds of J.curcas,and laid a foundation for further research on the pharmacodynamic substances and pharmacological mechanism of J.curcas.

关 键 词：麻风树 化学成分 BV-2小胶质细胞 一氧化氮 

分 类 号：R28[医药卫生—中药学]