人参皂苷Rg3对大鼠实验性脉络膜新生血管的影响及作用机制研究  

The Effects and Mechanisms of Ginsenoside Rg3 on Experimental Choroidal Neovas⁃cularization in Rats

作　　者：褚文丽 亢泽峰[2] 李书娇 侯昕玥 陈水龄 CHU Wenli;KANG Zefeng;LI Shujiao;HOU Xinyue;CHEN Shuiling(Bei-jing Longfu Hospital,Beijing 100010,China)

机构地区：[1]北京市隆福医院,北京100010 [2]中国中医科学院眼科医院,北京100040 [3]中国中医科学院西苑医院,北京100091

出　　处：《中国中医眼科杂志》2023年第8期701-706,共6页China Journal of Chinese Ophthalmology

基　　金：国家自然科学基金项目(81574032);中医药传承与创新“百千万”人才工程(岐黄工程)岐黄学者。

摘　　要：目的观察人参皂苷Rg3对大鼠实验性脉络膜新生血管(CNV)形成的干预作用,以及对缺氧诱导因子-1α(HIF-1α)和血管内皮生长因子(VEGF)表达的影响。方法30只大鼠经532 nm激光光凝建立CNV模型,被随机分为模型组(MG)、人参皂苷Rg3低剂量组(L-Rg3)、人参皂苷Rg3中剂量组(M-Rg3)、人参皂苷Rg3高剂量组(H-Rg3)、阳性对照药康柏西普组(CB),另设对照组(CG),每组6只。L-Rg3、M-Rg3、H-Rg3组分别予3.65、7.20、14.40 mg/kg的人参皂苷Rg3药液灌胃,而CG、MG组大鼠灌胃2 mL蒸馏水,每日1次,连续干预7 d;CB组于造模后一次性玻璃体腔注射康柏西普注射液5μL。给药完成后荧光素眼底血管造影(FFA)观察大鼠眼底情况,实时荧光定量PCR(RT-qPCR)检测脉络膜HIF-1α、VEGF mRNA表达,Western Blot法检测脉络膜HIF-1α、VEGF蛋白表达。结果(1)FFA:与CG组比较,MG组荧光素渗漏平均光密度(MD)值升高(t=17.846,P=0.000);与MG组比较,M-Rg3、H-Rg3、CB组MD值降低(t_(M-Rg3)=5.032,t_(H-Rg3)=6.593,t_(CB)=9.618,均P=0.000);与H-Rg3组比较,CB组MD值升高(t=3.025,P=0.011),差异均有统计学意义。(2)脉络膜HIF-1α表达:与CG组比较,MG组HIF-1αmRNA(t=16.280,P=0.000)和蛋白(t=14.406,P=0.000)表达升高;与MG组比较,M-Rg3、H-Rg3、CB组HIF-1αmRNA(t_(M-Rg3)=8.692,t_(H-Rg3)=12.070,t_(CB)=14.510,均P=0.000)和蛋白(t_(M-Rg3)=5.488,t_(H-Rg3)=8.918,t_(CB)=8.918,均P=0.000)表达降低;与CB组比较,H-Rg3组HIF-1αmRNA表达升高(t=2.431,P=0.031),差异均有统计学意义。(3)脉络膜VEGF表达:与CG组比较,MG组VEGF mRNA(t=14.500,P=0.000)和蛋白(t=17.938,P=0.000)表达升高;与MG组比较,M-Rg3、H-Rg3、CB组VEGF mRNA(t_(M-Rg3)=6.730,t_(H-Rg3)=10.068,t_(CB)=13.351,均P=0.000)和蛋白(t_(M-Rg3)=5.382,t_(H-Rg3)=8.969,t_(CB)=12.557,均P=0.000)表达降低;与CB组比较,H-Rg3组VEGF mRNA(t=3.283,P=0.007)和蛋白(t=3.588,P=0.004)表达升高,差异均有统计学意义。结论人参皂苷Rg3对大鼠实验性CNV具有抑制作用,其作用机制可能�OBJECTIVE To observe the intervention effects of Ginsenoside Rg3 on experimental choroidal neovascularization(CNV)in rats and its influence on the expression of hypoxiainducible factor-1α(HIF-1α)and vascular endothelial growth factor(VEGF).METHODS A total of 30 rats were used to establish the CNV model through 532 nm laser photocoagulation and were randomly divided into the model group(MG),low-dose Ginsenoside Rg3 group(L-Rg3),medium-dose Ginsenoside Rg3 group(M-Rg3),high-dose Ginsenoside Rg3 group(H-Rg3),positive control conbercept group(CB),and a control group(CG),with six rats in each group.L-Rg3,M-Rg3,and H-Rg3 were administered orally with 3.65,7.20,and 14.40 mg/kg of Ginsenoside Rg3 solution,respectively,while the CG and MG were orally administered with 2 mL of distilled water once daily for seven consecutive days.The CB received a single intravitreal injection of conbercept injection(5μL)after the model was established.Fundus fluorescein angiography(FFA)was conducted after medicine administration to observe the condition of the rat fundus.Real-time quantitative PCR(RT-qPCR)was used to detect the mRNA expression of HIF-1αand VEGF in the choroid,and Western Blot was performed to measure the protein expression of HIF-1αand VEGF.RESULTS(1)FFA:Compared to the CG,the mean density(MD)value in the MG increased(t=17.846,P=0.000).Compared to the MG,the MD values decreased in the M-Rg3,H-Rg3,and CB(t_(M-Rg3)=5.032,t_(H-Rg3)=6.593,t_(CB)=9.618,all P=0.000).Compared to the H-Rg3,the CB showed an increased MD value(t=3.025,P=0.011),with statistical significance.(2)HIF-1αexpression in the choroid:Compared to the CG,the expression of HIF-1αmRNA(t=16.280,P=0.000)and protein(t=14.406,P=0.000)increased in the MG.Compared to the MG,the expression of HIF-1αmRNA(t_(M-Rg3)=8.692,t_(H-Rg3)=12.070,t_(CB)=14.510,all P=0.000)and protein(t_(M-Rg3)=5.488,t_(H-Rg3)=8.918,t_(CB)=8.918,all P=0.000)decreased in the M-Rg3,H-Rg3,and CB.Compared to the CB,the H-Rg3 group showed an increased expression of HIF-1αmRNA(t=2.431,P=0.031),

关 键 词：人参皂苷RG3 脉络膜新生血管 缺氧诱导因子-1Α 血管内皮生长因子 

分 类 号：R285.5[医药卫生—中药学]