黄曲条跳甲的防治药剂筛选及其对药剂的季节敏感性研究  

Screening of pesticides for Phyllotreta striolata control and the seasonal sensitivity of this insect to pesticides

作　　者：徐嘉政 占绣萍[2] 田椿燕 赵卫中 须志平[1] Xu Jiazheng;Zhan Xiuping;Tian Chunyan;Zhao Weizhong;Xu Zhiping(Shanghai Key Laboratory of Chemical Biology,School of Pharmacy,East China University of Science and Technology,Shanghai 200237,China;Shanghai Agricultural Technology Extension Service Center,Shanghai 201103,China;Shanghai Shentian Agriculture Co.,Ltd.Shanghai 201700,China;Shanghai Shenggu Optoelectronic Technology Co.,Ltd.Shanghai 201600,China)

机构地区：[1]华东理工大学药学院/上海市化学生物学(芳香杂环)重点实验室,上海2002372 [2]上海市农业技术推广服务中心,上海201103 [3]上海申田农业有限公司,上海201700 [4]上海盛谷光电科技有限公司,上海201600

出　　处：《中国植保导刊》2023年第6期5-9,共5页China Plant Protection

基　　金：上海市科技兴农项目(2019-02-08-00-13-F01137);上海市青浦区科技发展基金农业项目(青科发2021-08);上海市青浦区产学研合作发展资金项目(青产学研2019-58)。

摘　　要：为筛选适于防治蔬菜种植地黄曲条跳甲的药剂,了解黄曲条跳甲对常用杀虫剂敏感性随季节变化的情况,开展试验测定15种药剂对黄曲条跳甲的毒力,并连续监测不同时期的黄曲条跳甲对杀虫剂的敏感性及其体内相关解毒代谢酶的活力变化。结果表明,辛硫磷对黄曲条跳甲的毒力最高,LC50为0.61 mg/L,溴氰虫酰胺、吡虫啉居次,LC50分别为17.59、17.69 mg/L,杀虫双、氟啶虫胺腈、呋虫胺和环氧虫啶也对其具较高杀虫活性。经监测发现,6月黄曲条跳甲对杀虫剂的敏感性最低,3月、9月和10月敏感性较为接近;其体内谷胱甘肽S-转移酶(GSTs)、多功能氧化酶(MFO)及羧酸酯酶(CarE)在不同月份的活性高低排序为6月>10月>9月>3月。在不同时期施药防治黄曲条跳甲需注意调整剂量,同时注意轮换使用不同作用机制的药剂,以延缓抗药性的发展。To screen suitable pesticides for the control of Phyllotreta striolata in vegetables and to understand the seasonal sensitivity of P.striolata to commonly used pesticides,the toxicity of 15 pesticides against P.striolata was determined.The seasonal sensitivity of P.striolata to different insecticides and the activity of related detoxification metabolic enzymes were continuously monitored in different periods.The results showed that phoxim had the highest toxicity against P.striolata,with an LC50 of 0.61 mg/L,followed by cyantraniliprole and imidacloprid with the LC50 of 17.59 and 17.69 mg/L,respectively.Bisultap,sulfoxaflor,dinotefuran,and cycloxaprid also exhibited relatively high insecticidal activity against P.striolata.In addition,the monitoring results revealed that the sensitivity of P.striolata to insecticides was the lowest in June,while the sensitivities were similar in March,September,and October.The activity of glutathione S-transferases(GSTs),multifunctional oxidases(MFOs),and carboxylesterases(CarEs)in P.striolata was ranked from high to low in June,October,September,and March.Based on the analysis,to delay the development of resistance,the dosage should be adjusted in different periods,and the pesticides with different modes of action should be used alternately.

关 键 词：杀虫剂 黄曲条跳甲 季节敏感性 酶活性 

分 类 号：S433.5[农业科学—农业昆虫与害虫防治]