A tetrahedral framework nucleic acid based multifunctional nanocapsule for tumor prophylactic mRNA vaccination  

作　　者：Yuhao Liu Songhang Li Shiyu Lin Sirong Shi Taoran Tian Bowen Zhang Tao Zhang Yunfeng Lin 

机构地区：[1]State Key Laboratory of Oral Diseases,National Clinical Research Center for Oral Diseases,West China Hospital of Stomatology,Sichuan University Chengdu 610041,China [2]Key Laboratory of Oral Biomedical Research of Zhejiang Province,Zhejiang University,Hangzhou 31000,China [3]Guanghua School of Stomatology,Guangdong Provincial Key Laboratory of Stomatology,Sun Yat-sen University,Guangzhou 510055,China

出　　处：《Chinese Chemical Letters》2023年第7期169-175,共7页中国化学快报（英文版）

基　　金：supported by National Key R&D Program of China (No. 2019YFA0110600);National Natural Science Foundation of China (No. 81970916);Sichuan Province Youth Science and Technology Innovation Team (No. 2022JDTD0021);Research Funding from West China School/Hospital of Stomatology Sichuan University (No. RCDWJS2021-20);China Postdoctoral Science Foundation (No. 2022TQ0381)。

摘　　要：Synthetic antigen-encoding mRNA plays an increasingly significant role in tumor vaccine technology owing to its antigen-specific immune-activation. However, its immune efficacy is challenged by inferior delivery efficiency and demand for suitable adjuvants. Here, we develop a novel mRNA nanovaccine based on a multifunctional nanocapsule, which is a dual-adjuvant formulation composed of cytosine-phosphateguanine motifs loaded tetrahedral framework nucleic acid(CpG-tFNA) and an immunopeptide murine β-defensin 2(mDF2β). This m RNA nanovaccine successfully achieves intracellular delivery, antigen expression and presentation of dendritic cells, and proliferation of antigen-specific T cells. In a tumor prophylactic vaccination model, it exerts an excellent inhibitory effect on lymphoma occurrence through cellular immunity. This mRNA nanovaccine has promising prophylactic applications in tumors and many other diseases.

关 键 词：Tetrahedral framework nucleic acid(tFNA) RNA nanovaccine Cytosine-phosphate-guanine(CpG)motifs Murineβ-defensin 2(mDF2β) Tumor immunotherapy 

分 类 号：TQ464[化学工程—制药化工] TB383.1[一般工业技术—材料科学与工程]