Electrochemical analysis of microRNAs with hybridization chain reaction-based triple signal amplification  

作　　者：Jianfeng Ma Lingbo Gong Yingying Cen Lin Feng Yan Su Xingfen Liu Jie Chao Ying Wan Shao Su Lianhui Wang 

机构地区：[1]State Key Laboratory of Organic Electronics and Information Displays&Jiangsu Key Laboratory for Biosensors,Institute of Advanced Materials(IAM).Nanjing University of Posts and Telecommunications,Nanjing 210023,China [2]School of Mechanical Engineering,Nanjing University of Science and Technology,Nanjing 210094,China

出　　处：《Chinese Chemical Letters》2023年第7期210-213,共4页中国化学快报（英文版）

基　　金：supported by the National Key Research and Development Program of China (No. 2017YFA0205302);the Natural Science Foundation of Jiangsu Province-Major Project (No. BK20212012);the National Natural Science Foundation of China (No. 21874071);the “Six Talents Peak” Foundation of Jiangsu Province (No. SWYY-046);the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD, No. YX030003)。

摘　　要：Selective and sensitive detection of trace microRNA is important for early diagnosis of diseases due to its expression level related to diseases.Herein,a triple signal amplification strategy is developed for trace microRNA-21 (miRNA-21) detection by combining with target-triggered cyclic strand displacement reaction (TCSDR),hybridization chain reaction (HCR) and enzyme catalytic amplification.Four DNA hairpins(H1,H2,H3,H4) are employed to form an ultralong double-strand DNA (dsDNA) structure,which is initiated by target miRNA-21.As H3 and H4 are labeled with horseradish peroxidase (HRP),numerous HRPs are loaded on the long dsDNA,producing significantly enhanced electrocatalytic signals in the hydrogen peroxide (H_(2)O_(2)) and 3,3,5,5-tetramethylbenzidine (TMB) reaction strategy.Compared with single signal amplification,the triple signal amplification strategy shows higher electrochemical response,wider dynamic range and lower detection limit for miRNA-21 detection with excellent selectivity,reproducibility and stability.Taking advantage of the triple signal amplification strategy,the proposed electrochemical biosensor can detect miRNA-21 in 10 He La cell lysates,suggesting that it is a promising method for fruitful assay in clinical diagnosis.

关 键 词：Electrochemical BIOSENSOR MICRORNAS Hybridization chain reaction Target-triggered cyclic strand displacement REACTION Triple signal amplification 

分 类 号：Q522[生物学—生物化学] O657.1[理学—分析化学]